Xiang Yuting, Liu Haitao, Yang Wenjian, Xu Zhongyuan, Wu Yue, Tang Zhaojian, Zhu Zhijing, Zeng Zhiyong, Wang Depeng, Wang Tianxing, Hu Ning, Zhang Diming
Department of Obstetrics and Gynecology, Affiliated Dongguan People's Hospital, Southern Medical University, Dongguan, 523058 China.
Research Center for Intelligent Sensing Systems, Zhejiang Laboratory, Hangzhou, 311100 China.
Microsyst Nanoeng. 2022 Jun 27;8:70. doi: 10.1038/s41378-022-00408-9. eCollection 2022.
Electrophysiological recording is a widely used method to investigate cardiovascular pathology, pharmacology and developmental biology. Microelectrode arrays record the electrical potential of cells in a minimally invasive and high-throughput way. However, commonly used microelectrode arrays primarily employ planar microelectrodes and cannot work in applications that require a recording of the intracellular action potential of a single cell. In this study, we proposed a novel measuring method that is able to record the intracellular action potential of a single cardiomyocyte by using a nanowell patterned microelectrode array (NWMEA). The NWMEA consists of five nanoscale wells at the center of each circular planar microelectrode. Biphasic pulse electroporation was applied to the NWMEA to penetrate the cardiomyocyte membrane, and the intracellular action potential was continuously recorded. The intracellular potential recording of cardiomyocytes by the NWMEA measured a potential signal with a higher quality (213.76 ± 25.85%), reduced noise root-mean-square (~33%), and higher signal-to-noise ratio (254.36 ± 12.61%) when compared to those of the extracellular recording. Compared to previously reported nanopillar microelectrodes, the NWMEA could ensure single cell electroporation and acquire high-quality action potential of cardiomyocytes with reduced fabrication processes. This NWMEA-based biosensing system is a promising tool to record the intracellular action potential of a single cell to broaden the usage of microelectrode arrays in electrophysiological investigation.
电生理记录是一种广泛用于研究心血管病理学、药理学和发育生物学的方法。微电极阵列以微创和高通量的方式记录细胞的电势。然而，常用的微电极阵列主要采用平面微电极，无法在需要记录单个细胞胞内动作电位的应用中使用。在本研究中，我们提出了一种新颖的测量方法，即使用纳米阱图案化微电极阵列（NWMEA）来记录单个心肌细胞的胞内动作电位。NWMEA由每个圆形平面微电极中心的五个纳米级阱组成。对NWMEA施加双相脉冲电穿孔以穿透心肌细胞膜，并持续记录胞内动作电位。与细胞外记录相比，NWMEA对心肌细胞的胞内电位记录测量到的电位信号质量更高（213.76 ± 25.85%），噪声均方根降低（约33%），信噪比更高（254.36 ± 12.61%）。与先前报道的纳米柱微电极相比，NWMEA可以确保单细胞电穿孔，并通过减少制造工艺获得高质量的心肌细胞动作电位。这种基于NWMEA的生物传感系统是一种很有前景的工具，可用于记录单个细胞的胞内动作电位，以拓宽微电极阵列在电生理研究中的应用。
