在不同水合条件下对胚胎进行活体成像。
Live imaging embryos under different hydration conditions.
机构信息
Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA.
Department of Biology, Stanford University, Stanford, CA 94305, USA.
出版信息
STAR Protoc. 2021 Dec 13;2(4):101025. doi: 10.1016/j.xpro.2021.101025. eCollection 2021 Dec 17.
Despite the ecological and agronomical importance of seed germination, how seeds integrate environmental signals to trigger germination remains enigmatic. Recently we reported that a protein called FLOE1 is involved in sensing and responding to water availability during germination. Here, we present a live-imaging protocol to assess the subcellular localization of a protein of interest during imbibition of desiccated seeds with the goal of understanding protein dynamics during the early stages of water uptake. For complete details on the use and execution of this profile, please refer to Dorone et al. (2021).
尽管种子萌发具有生态和农艺学重要性，但种子如何整合环境信号以触发萌发仍然是个谜。最近，我们报道了一种叫做 FLOE1 的蛋白质参与感知和响应萌发过程中水分的可用性。在这里，我们提出了一种活细胞成像方案，以评估在干燥种子吸水过程中目标蛋白的亚细胞定位，旨在了解蛋白在吸水早期的动态变化。有关该方案使用和执行的完整详细信息，请参考 Dorone 等人（2021 年）。
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