L'Oréal Research & Innovation, Chevilly Larue, France.
Laboratoires Vichy, Levallois Perret, France.
J Eur Acad Dermatol Venereol. 2022 Jan;36 Suppl 2:16-25. doi: 10.1111/jdv.17786.
Probiotics are live microorganisms, which, when administered in adequate amounts, confer a health benefit on the host. Semiactive, non-replicating bacteria or extracts used in dermocosmetics have interesting properties for skin quality. Vitreoscilla filiformis is cultured by a fermentation process to obtain an extract. It is considered as a probiotic fraction and topical application of this extract has shown activity to strengthen the skin physical barrier function and maintain good homeostasis of skin defenses. Vichy volcanic mineralizing water (VVMW) is a pure, highly mineralized water that has been shown to strengthen the skin against exposome aggressions. This manuscript reviews properties of probiotic fractions used in skin care, especially studies on an extract of V. filiformis grown in a medium containing VVMW (VfeV) and evaluated in combination with VVMW. Skin barrier function: In normal human epidermal keratinocyte cultures, the combination of 10% VVMW and 0.002% VfeV significantly increased transglutaminase, filaggrin, involucrin, claudin-1, and zonula occludens-1 in comparison with the controls. Antimicrobial peptide defenses: The combination of 16.7% VVMW and 0.1% VfeV increased the expression of β-defensin-4A and S100A7. Skin immune defense functions: In lipopolysaccharide-stimulated peripheral blood mononuclear cells, the combination of 16.7% VVMW and 0.1% VfeV down-regulated IL-8, TNF-α, IL-12/IL-23p40, and increased IL10 and IL-10/IL-12 ratio compared to the control. Additionally, the combination of 79% VVMW plus 5% VfeV protected Langerhans cells in skin explants exposed to ultraviolet radiation. In conclusion, the combination of VfeV plus VVMW has properties to strengthen the skin barrier by stimulating skin differentiation and tight junctions, biochemical defenses by stimulating antimicrobial peptides, and cellular immune defenses by increasing the IL-10/IL-12 ratio and by protecting Langerhans cells challenged by ultraviolet radiation.
益生菌是活的微生物，当以足够的量使用时，会给宿主带来健康益处。在皮肤化妆品中使用半活的、非复制的细菌或提取物具有改善皮肤质量的有趣特性。丝状威斯克菌通过发酵过程培养以获得提取物。它被认为是一种益生菌分数，局部应用这种提取物已显示出增强皮肤物理屏障功能和维持皮肤防御良好平衡的活性。Vichy 火山矿泉水（VVMW）是一种纯净、高矿物质的水，已被证明可增强皮肤对暴露组攻击的抵抗力。本文综述了用于皮肤护理的益生菌分数的特性，特别是关于在含有 VVMW 的培养基中生长的丝状威斯克菌提取物（VfeV）的研究，并与 VVMW 联合评估。皮肤屏障功能：在正常的人表皮角质形成细胞培养物中，与对照相比，10%VVMW 和 0.002%VfeV 的组合显著增加了转谷氨酰胺酶、丝聚蛋白、内披蛋白、闭合蛋白-1 和封闭蛋白-1。抗菌肽防御：16.7%VVMW 和 0.1%VfeV 的组合增加了β-防御素-4A 和 S100A7 的表达。皮肤免疫防御功能：在脂多糖刺激的外周血单核细胞中，与对照相比，16.7%VVMW 和 0.1%VfeV 的组合下调了 IL-8、TNF-α、IL-12/IL-23p40，并增加了 IL10 和 IL-10/IL-12 比值。此外，79%VVMW 加 5%VfeV 的组合保护了暴露于紫外线辐射的皮肤外植体中的朗格汉斯细胞。总之，VfeV 加 VVMW 的组合通过刺激皮肤分化和紧密连接来增强皮肤屏障，通过刺激抗菌肽来增强生化防御，通过增加 IL-10/IL-12 比值和保护朗格汉斯细胞免受紫外线辐射来增强细胞免疫防御。
