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利用 DNA 条形码和比较转录组学鉴定野葛属植物。

Identification of Pueraria spp. through DNA barcoding and comparative transcriptomics.

机构信息

BioDiscovery Institute and Department of Biological Sciences, University of North Texas, 1155 Union Circle #305220, Denton, TX, 76203-5017, USA.

College of Life Sciences, Hubei University, Wuhan, 430068, Hubei Province, China.

出版信息

BMC Plant Biol. 2022 Jan 3;22(1):10. doi: 10.1186/s12870-021-03383-x.

DOI:10.1186/s12870-021-03383-x
PMID:34979934
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8722073/
Abstract

BACKGROUND

Kudzu is a term used generically to describe members of the genus Pueraria. Kudzu roots have been used for centuries in traditional Chinese medicine in view of their high levels of beneficial isoflavones including the unique 8-C-glycoside of daidzein, puerarin. In the US, kudzu is seen as a noxious weed causing ecological and economic damage. However, not all kudzu species make puerarin or are equally invasive. Kudzu remains difficult to identify due to its diverse morphology and inconsistent nomenclature.

RESULTS

We have generated sequences for the internal transcribed spacer 2 (ITS2) and maturase K (matK) regions of Pueraria montana lobata, P. montana montana, and P. phaseoloides, and identified two accessions previously used for differential analysis of puerarin biosynthesis as P. lobata and P. phaseoloides. Additionally, we have generated root transcriptomes for the puerarin-producing P. m. lobata and the non-puerarin producing P. phaseoloides. Within the transcriptomes, microsatellites were identified to aid in species identification as well as population diversity.

CONCLUSIONS

The barcode sequences generated will aid in fast and efficient identification of the three kudzu species. Additionally, the microsatellites identified from the transcriptomes will aid in genetic analysis. The root transcriptomes also provide a molecular toolkit for comparative gene expression analysis towards elucidation of the biosynthesis of kudzu phytochemicals.

摘要

背景

葛根是指豆科葛属的植物。葛根在传统中药中已有数百年的使用历史，因其含有高水平的有益异黄酮，包括独特的大豆苷元 8-C-糖苷、葛根素。在美国，葛根被视为有害杂草，造成生态和经济破坏。然而，并非所有的葛属植物都能产生葛根素，也并非所有植物都同样具有入侵性。由于其形态多样且命名不一致，葛根仍然难以识别。

结果

我们已经生成了野葛、野葛和葛藤的内转录间隔区 2（ITS2）和成熟酶 K（matK）区域的序列，并鉴定了两个先前用于葛根素生物合成差异分析的材料，即野葛和葛藤。此外，我们还生成了产葛根素的野葛和不产葛根素的葛藤的根转录组。在转录组中，鉴定出微卫星以帮助物种鉴定和种群多样性分析。

结论

生成的条形码序列将有助于快速、有效地鉴定这三种葛属植物。此外，从转录组中鉴定出的微卫星将有助于遗传分析。根转录组还为比较基因表达分析提供了一个分子工具包，以阐明葛根植物化学物质的生物合成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/962a/8722073/74f3152fb115/12870_2021_3383_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/962a/8722073/f8b8385c6736/12870_2021_3383_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/962a/8722073/9890330b1777/12870_2021_3383_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/962a/8722073/07a9b097d359/12870_2021_3383_Fig5_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/962a/8722073/74f3152fb115/12870_2021_3383_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/962a/8722073/f8b8385c6736/12870_2021_3383_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/962a/8722073/80a856a3e096/12870_2021_3383_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/962a/8722073/9d5a7f20e535/12870_2021_3383_Fig3_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/962a/8722073/07a9b097d359/12870_2021_3383_Fig5_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/962a/8722073/74f3152fb115/12870_2021_3383_Fig7_HTML.jpg

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2
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3
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DNA Res. 2024 Jun 1;31(3). doi: 10.1093/dnares/dsae017.
4
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5
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5
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