West China Hospital, Sichuan University, Chengdu, Sichuan, China.
West China School of Medicine, Sichuan University, Chengdu, Sichuan, China.
BMC Nephrol. 2022 Jan 3;23(1):10. doi: 10.1186/s12882-021-02638-x.
The pathogenesis of Henoch-Schönlein purpura nephritis (HSPN) is closely associated with mucosal infection. But whether intestinal microbiota dysbiosis plays a role in it is not clear.
A total of 52 participants including 26 HSPN patients and 26 healthy controls were included. By using 16S ribosomal RNA gene sequencing, the intestinal microbiota composition between HSPN and healthy controls was compared. The diagnostic potency was evaluated by Receiver operating characteristic (ROC) with area under curves (AUC). Meanwhile, correlation analysis was also performed.
The lower community richness and diversity of fecal microbiota was displayed in HSPN patients and the structure of gut microbiota was remarkedly different. A genus-level comparison indicated a significant increase in the proportions of g-Bacteroides, g-Escherichia-Shigella and g-Streptococcus, and a marked reduction of g-Prevotella_9 in HSPN patients, suggesting that the overrepresentation of potential pathogens and reduction of profitable strains were the main feature of the dysbiosis. The differential taxonomic abundance might make sense for distinguishing HSPN from healthy controls, with AUC of 0.86. The relative abundance of the differential bacteria was also concerned with clinical indices. Among them, Streptococcus spp. was positively associated with the severity of HSPN (P < 0.050). It was found that HSPN patients with higher level of Streptococcus spp. were more likely to suffering from hematuria and hypoalbuminemia (P < 0.050).
The dysbiosis of gut microbiota was obvious in HSPN patients, and the intestinal mucosal streptococcal infection was distinctive, which was closely related to its severity.
过敏性紫癜肾炎（HSPN）的发病机制与黏膜感染密切相关。但肠道微生物群失调是否在其中起作用尚不清楚。
共纳入 52 名参与者，包括 26 名 HSPN 患者和 26 名健康对照者。采用 16S 核糖体 RNA 基因测序，比较 HSPN 与健康对照组之间的肠道微生物组成。采用接收者操作特征（ROC）曲线评估诊断效能，曲线下面积（AUC）。同时进行相关性分析。
HSPN 患者粪便微生物群落丰富度和多样性较低，肠道微生物群结构明显不同。属水平比较表明，g-Bacteroides、g-Escherichia-Shigella 和 g-Streptococcus 的比例显著增加，g-Prevotella_9 的比例明显降低，提示潜在致病菌的过度表达和有益菌株的减少是菌群失调的主要特征。分类丰度的差异可能有助于区分 HSPN 与健康对照组，AUC 为 0.86。差异细菌的相对丰度也与临床指标有关。其中，链球菌属与 HSPN 的严重程度呈正相关（P<0.050）。研究发现，链球菌属水平较高的 HSPN 患者更易出现血尿和低白蛋白血症（P<0.050）。
HSPN 患者肠道微生物群失调明显，肠道黏膜链球菌感染具有特征性，与疾病严重程度密切相关。
