Department of Pediatrics, Affiliated Dongfang Hospital, Xiamen University, Fuzhou, China.
Department of Pediatrics, The 900th Hospital of Joint Logistic Support Force, Fuzhou, China.
Front Cell Infect Microbiol. 2021 Jul 1;11:641997. doi: 10.3389/fcimb.2021.641997. eCollection 2021.
Gut microbiota plays an important role in the pathogenesis of immune-mediated diseases. However, the complex pathogenesis of Henoch-Schonlein Purpura (HSP) remains elusive. This study aimed to characterize the gut microbiota in HSP patients and explore the potential association between gut microbiota composition and phenotypic changes in HSP.
16SrRNA gene sequencing and bioinformatic analyses were performed using total DNA extracted from the fecal microbiota of 34 children with HSP, including 18 primary cases, 16 recurrent cases, and 23 healthy children.
The diversity indexes showed significant differences in the microbial community among the primary HSP groups, the recurrent HSP group and healthy controls. The abundance of in the recurrent HSP group was significantly higher than that in the primary HSP group, and the constructed ROC curve had an AUC value of 0.750. According to the Spearman correlation analysis, the abundance of was positively associated with the serum IgG level in children with HSP, while the abundance of was negatively correlated with the complement component 3 (C3). The diversity indexes of gut microbiota in the HSP group with abdominal symptoms were higher than those in the HSP group without GI involvement, and also higher than those in the healthy control group. In the HSP group with GI involvement, the abundance of was decreased, while the abundance of and was increased, compared to the HSP group without GI involvement.
The gut microbiota of children with HSP was different from that of healthy children. The genus has a diagnostic value for HSP recurrence. and may affect IgG and complement C3 levels in children with HSP. Abdominal symptoms in HSP children were related to gut microbiota ( and butyric acid-producing bacteria).
肠道微生物群在免疫介导性疾病的发病机制中起着重要作用。然而，过敏性紫癜（HSP）的复杂发病机制仍不清楚。本研究旨在描述 HSP 患者的肠道微生物群，并探讨肠道微生物群组成与 HSP 表型变化之间的潜在关联。
使用从 34 名 HSP 患儿的粪便微生物群中提取的总 DNA 进行 16SrRNA 基因测序和生物信息学分析，包括 18 名初发病例、16 名复发病例和 23 名健康对照儿童。
初发 HSP 组、复发 HSP 组和健康对照组之间的微生物群落多样性指数存在显著差异。复发 HSP 组中 的丰度明显高于初发 HSP 组，构建的 ROC 曲线 AUC 值为 0.750。根据 Spearman 相关性分析，HSP 患儿血清 IgG 水平与 的丰度呈正相关，而补体成分 3（C3）与 的丰度呈负相关。有腹部症状的 HSP 组的肠道微生物多样性指数高于无胃肠道受累的 HSP 组，也高于健康对照组。在有胃肠道受累的 HSP 组中，与无胃肠道受累的 HSP 组相比， 的丰度降低，而 和 的丰度增加。
HSP 患儿的肠道微生物群与健康儿童不同。属 对 HSP 复发有诊断价值。和 可能影响 HSP 患儿 IgG 和补体 C3 水平。HSP 患儿的腹部症状与肠道微生物群（和丁酸产生菌）有关。
