Kaufmann Y, Silverman T, Levi B Z, Ozato K
J Exp Med. 1987 Sep 1;166(3):810-5. doi: 10.1084/jem.166.3.810.
Expression of cellular oncogenes was studied in a T cell hybridoma that undergoes cytolytic activation when stimulated by specific antigen or by anti-Thy-1 antibody. The activation occurs without induction of hybridoma proliferation, providing a model to examine oncogene expression during functional differentiation of lymphocytes. We found that c-fos and c-ets-1 mRNAs were transiently induced at high levels in the hybridoma 30 min and 4 h after stimulation, respectively. c-myc and c-ets-2 oncogenes were constitutively expressed in the hybridoma and their mRNA levels were unaffected during 4 h of stimulation, although c-myc expression was reduced in the later stage of stimulation. Inhibitors of T cell activation, cyclosporin A and anti-LFA-1 antibody, blocked the induction of c-fos and c-ets-1 mRNAs without reducing the levels of c-myc and c-ets-2. The results indicate that the functional activation of the CTL hybridoma is associated with induction of c-fos and c-ets-1 genes.
在一种T细胞杂交瘤中研究了细胞癌基因的表达，该杂交瘤在受到特异性抗原或抗Thy-1抗体刺激时会发生溶细胞激活。这种激活在不诱导杂交瘤增殖的情况下发生，为研究淋巴细胞功能分化过程中的癌基因表达提供了一个模型。我们发现，c-fos和c-ets-1 mRNA分别在刺激后30分钟和4小时在杂交瘤中被短暂地高水平诱导。c-myc和c-ets-2癌基因在杂交瘤中组成性表达，其mRNA水平在4小时的刺激过程中未受影响，尽管c-myc表达在刺激后期有所降低。T细胞激活抑制剂环孢素A和抗LFA-1抗体可阻断c-fos和c-ets-1 mRNA的诱导，而不降低c-myc和c-ets-2的水平。结果表明，CTL杂交瘤的功能激活与c-fos和c-ets-1基因的诱导有关。
