Walter M, Brenner U, Holzmüller W, Müller J M
Lab Anim. 1987 Jul;21(3):195-200. doi: 10.1258/002367787781268828.
A new preparation process was studied which should allow the implantation of collagen type I in its native structure in reconstructive surgery, in this special case for closure of incisional hernias. As experimental animals we used 30 female Lewis rats. A defect of the anterior abdominal wall measuring 3 cm X 4 cm was closed with our collagen substitute. Biopsies taken after 4, 6 and 8 weeks were examined morphologically. As criteria for revitalization and revascularization we used the type of infiltrating cells, the depth and density of infiltration and the formation of new blood vessels. After 4 weeks the implants were infiltrated by fibroblasts that decreased in density towards the centre. Good revascularization could be seen on the muscle-implant interface. After 6 weeks the density of infiltrating cells had increased markedly even to the centre of the collagen implant. Sporadically small vessels could be seen. Eight weeks after implantation the density of infiltrated cells was at the same high level, and capillary bundles could be seen within the whole implant. We believe that this collagen implant is suitable for the closure of hernias as shown by its physical and morphological properties. In particular it appears to guarantee and earlier and tighter closure of hernias than other materials.
研究了一种新的制备工艺，该工艺应能使I型胶原蛋白以其天然结构植入重建手术中，在这种特殊情况下用于闭合切口疝。我们使用30只雌性Lewis大鼠作为实验动物。用我们的胶原蛋白替代物闭合3厘米×4厘米的前腹壁缺损。在4周、6周和8周后取活检进行形态学检查。我们将浸润细胞的类型、浸润的深度和密度以及新血管的形成作为活力化和血管化的标准。4周后，植入物被成纤维细胞浸润，其密度向中心降低。在肌肉-植入物界面可见良好的血管化。6周后，浸润细胞的密度显著增加，甚至到胶原蛋白植入物的中心。偶尔可见小血管。植入8周后，浸润细胞的密度保持在相同的高水平，并且在整个植入物内可见毛细血管束。我们认为，这种胶原蛋白植入物适合用于闭合疝，如其物理和形态学特性所示。特别是，它似乎比其他材料能保证更早、更紧密地闭合疝。
