Walter M, Brenner U, Keller H W, Müller J M
Langenbecks Arch Chir. 1985;363(4):253-60. doi: 10.1007/BF01262499.
A new preparation process was studied which should allow the implantation of collagen type I in its native structure in reconstructive surgery, in this special case for closure of incisional hernias. As experimental animals we used 30 female Lewis rats. A defect of the anterior abdominal wall - measuring 3 X 4 cm - was closed with our collagen substitute. Biopsies were taken after 4, 6 and 8 weeks and examined morphologically. As criteria for revitalization and revascularisation we used type of immigrated cells, depth and density of immigration and formation of new blood vessels. After 4 weeks the implants were immigrated by fibroblasts, density diminishing towards the centre. On the muscle-implant-interface good revascularisation could be seen. After 6 weeks density of immigrated cells has distinctly increased even in the centre of the collagen implant. Sporadically small vessels could be seen. Eight weeks after implantation density of immigrated cells was at the same high level, and capillary bundles could be seen within the whole implant. We think that the studied collagen implant is suitable for the closure of hernias as shown by its physical and morphological properties. Especially it seems to guarantee an earlier and tighter closure of hernias in comparison to other materials.
研究了一种新的制备工艺，该工艺应能使I型胶原蛋白以其天然结构植入重建手术中，在这种特殊情况下用于闭合切口疝。我们使用30只雌性Lewis大鼠作为实验动物。用我们的胶原蛋白替代物闭合3×4厘米的前腹壁缺损。在4周、6周和8周后取活检并进行形态学检查。作为活化和血管再生的标准，我们使用迁移细胞的类型、迁移深度和密度以及新血管的形成。4周后，植入物有成纤维细胞移入，密度向中心递减。在肌肉-植入物界面可见良好的血管再生。6周后，即使在胶原蛋白植入物的中心，移入细胞的密度也明显增加。偶尔可见小血管。植入8周后，移入细胞的密度处于相同的高水平，并且在整个植入物中可见毛细血管束。我们认为，所研究的胶原蛋白植入物适合用于闭合疝，这从其物理和形态学特性中可以看出。特别是与其他材料相比，它似乎能保证更早、更紧密地闭合疝。
