Laboratory of Plant Healthcare and Diagnostics, PG Department of Studies in Biotechnology and Microbiology, Karnatak University, Pavate Nagar, Dharwad, 580 003, Karnataka, India.
Laboratory of Plant Healthcare and Diagnostics, PG Department of Studies in Biotechnology and Microbiology, Karnatak University, Pavate Nagar, Dharwad, 580 003, Karnataka, India; Division of Biological Sciences, School of Science and Technology, University of Goroka, Goroka, 441, Papua New Guinea.
J Biotechnol. 2021 Jan 10;325:196-206. doi: 10.1016/j.jbiotec.2020.10.023. Epub 2020 Oct 23.
With the advent of rapid evolution of oomycete pathogen lineages, the need for sustainable agriculture practices has become the need of the hour. The late blight of tomato caused by Phytopthora infestans, has recently emerged as one such devastating disease in India that led to huge crop losses. Hence, in the present work seed priming with mycogenic selenium nanoparticles (SeNPs) for elicitation of resistance against tomato late blight disease is investigated. It also aims to understand the defense responses triggered by SeNPs at cellular, biochemical and transcriptomic levels. Enhanced plant growth parameters were observed in bioactive SeNPs-primed tomato plants as compared to control plants. SeNPs-primed and pathogen inoculated plants exhibited a significant protection of 72.9 % against late blight disease. The primed plants also recorded a remarkable accumulation of lignin, callose and hydrogen peroxide that serve as the cellular defense over the control plants. Further, an elevated level of lipoxygenase (LOX), phenylalanine lyase (PAL), β-1,3-glucanase (GLU), superoxide dismutase (SOD) corroborated the biochemical defense in primed plants, which was also reflected in the corresponding transcriptome profiling of the genes encoding the enzymes. Thus, the present study represents an orchestrated correlation between resistance and defense responses incited by SeNPs against tomato late blight disease, which can be used as nano-biostimulant fungicide in protecting tomato plants.
随着卵菌病原体谱系的快速进化，可持续农业实践的需求已成为当务之急。由致病疫霉引起的番茄晚疫病最近在印度成为一种破坏性很强的疾病，导致了巨大的作物损失。因此，本研究采用真菌源硒纳米颗粒（SeNPs）对番茄晚疫病进行种予引发处理，以诱导抗性。本研究还旨在从细胞、生化和转录组水平上了解 SeNPs 引发的防御反应。与对照植株相比，生物活性 SeNPs 引发的番茄植株的生长参数得到了增强。与对照植株相比，SeNPs 引发和病原菌接种的植株对晚疫病的保护率达到了 72.9%。引发植株还记录到木质素、胼胝质和过氧化氢的显著积累，这些物质作为细胞防御物质，超过了对照植株。此外，脂氧合酶（LOX）、苯丙氨酸解氨酶（PAL）、β-1,3-葡聚糖酶（GLU）和超氧化物歧化酶（SOD）的水平升高，证实了引发植株中的生化防御，这也反映在编码这些酶的基因的相应转录组谱中。因此，本研究代表了 SeNPs 对番茄晚疫病引发的抗性和防御反应之间的协调关联，可作为保护番茄植株的纳米生物刺激素杀菌剂。
