Qinghai Provincial Key Laboratory of Tibetan Medicine Research and Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, CAS, Xining 810001, China.
Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, CAS, Beijing 100093, China.
Food Funct. 2022 Jan 24;13(2):846-856. doi: 10.1039/d1fo03412j.
Tree peonies are well-known horticultural and medicinal plants. The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities. In this study, the phytochemicals isolated from tree peony seed coats were systematically investigated. Seven polyphenolics were separated and prepared, mainly belonging to resveratrol derivatives. There was a great variation in the seed coat polyphenolic content among eight species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of than other species. Based on the HPLC fingerprint characteristics and chemometric analysis, a clear discrimination among plants was found, including the composition patterns and contents of the constituents. Moreover, the characteristic phytochemicals (vateriferol and -ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice. In addition, enzyme tests showed that the two compounds could effectively and competitively inhibit α-glucosidase, with the IC values of 3.01 and 7.75 μM, respectively, indicating that vateriferol and -ε-viniferin could be therapeutic potential agents for hyperglycemia and diabetes mellitus.
牡丹是著名的园艺和药用植物。近年来，作为新兴木本油料作物的牡丹籽因其代谢产物和生物活性而备受关注。本研究系统地研究了牡丹种皮中分离得到的植物化学物质。从牡丹种皮中分离并制备了 7 种多酚类化合物，主要属于白藜芦醇衍生物。8 种牡丹种皮中多酚类化合物含量差异较大， 种皮中白藜芦醇三聚体和二聚体含量明显高于其他种。基于 HPLC 指纹特征和化学计量学分析，发现 8 种植物之间存在明显的区分，包括成分的组成模式和含量。此外，特征性植物化学物质（芒柄花苷和 -ε-viniferin）可显著降低糖尿病/正常小鼠餐后血糖水平。此外，酶试验表明，这两种化合物可有效且竞争性地抑制 α-葡萄糖苷酶，IC 值分别为 3.01 和 7.75 μM，表明芒柄花苷和 -ε-viniferin可能是治疗高血糖和糖尿病的潜在药物。
