Dokuz Eylul University, Department of Physiology, School of Medicine, Izmir, Turkey; Dokuz Eylul University, School of Sport Sciences and Technology, Izmir, Turkey.
Dokuz Eylul University, Department of Physiology, School of Medicine, Izmir, Turkey; Dokuz Eylül University, Department of Physiology, Graduate School of Health Sciences, Izmir, Turkey.
Neurosci Lett. 2022 Jan 23;770:136443. doi: 10.1016/j.neulet.2021.136443. Epub 2022 Jan 3.
The positive effects of both ketogenic diet (KD) and regular voluntary exercise on anxiety and depression behavior have been recently reported in rodent animals, but the effects of pairing a KD with exercise on depression and anxiety are unknown. In this study, we aimed to investigate the effects of combination of KD and regular voluntary exercise on anxiety and depression-like behavior in Balb/c mice. We've demostrated that anxiety and depression levels decreased in KD-exercised (KD-Ex) mice. β-hydroxybutyrate (BHB) levels increased while glucose, insulin levels and LDL/HDL ratio decreased in KD-Ex mice. There was a negative correlation between BHB and the time spent in the closed arms of elevated plus maze (EPM) or the time spent in periphery walls of open field test (OFT) and the immobility time in forced swim test (FST) which all of them are indicators of low depression and anxiety levels. There was a positive correlation between LDL/HDL ratio and the time spent in the closed arms of EPM or the immobility time in FST. The immobility time in FST was positively correlated with insulin while the mobility time in FST was negatively correlated with glucose. In conclusion, these results suggest that decline in anxiety and depression-like behaviors resulted from KD with regular voluntary exercise may be associated with increased BHB levels and decreased LDL/HDL ratio and insulin or glucose levels. Further research is necessary for our understanding of the mechanisms by which pairing a KD with voluntary exercise influences brain and behavior.
生酮饮食（KD）和常规自愿运动对焦虑和抑郁行为的积极影响最近在啮齿动物中报道，但将 KD 与运动相结合对抑郁和焦虑的影响尚不清楚。在这项研究中，我们旨在研究 KD 和常规自愿运动相结合对 Balb/c 小鼠焦虑和抑郁样行为的影响。我们已经证明，KD 运动（KD-Ex）小鼠的焦虑和抑郁水平降低。KD-Ex 小鼠的β-羟丁酸（BHB）水平升高，而葡萄糖、胰岛素水平和 LDL/HDL 比值降低。BHB 与高架十字迷宫（EPM）封闭臂停留时间或旷场试验（OFT）外周壁停留时间和强迫游泳试验（FST）的不动时间呈负相关，所有这些指标均表明抑郁和焦虑水平较低。LDL/HDL 比值与 EPM 封闭臂停留时间或 FST 不动时间呈正相关。FST 中的不动时间与胰岛素呈正相关，而 FST 中的移动时间与葡萄糖呈负相关。总之，这些结果表明，KD 结合常规自愿运动导致的焦虑和抑郁样行为的下降可能与 BHB 水平升高、LDL/HDL 比值降低以及胰岛素或葡萄糖水平降低有关。为了我们理解 KD 与自愿运动相结合如何影响大脑和行为，需要进一步的研究。
