Okrathok Supattra, Thumanu Kanjana, Pukkung Chayanan, Molee Wittawat, Khempaka Sutisa
School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima, 30000, Thailand.
Anim Biosci. 2022 Jul;35(7):1048-1058. doi: 10.5713/ab.21.0430. Epub 2022 Jan 5.
The present study was to investigate the extraction conditions of dietary fiber from dried cassava pulp (DCP) and cassava distiller's dried grains (CDG) under different NaOH concentrations, and the Fourier transform infrared (FTIR) was used to determine the dietary fiber components.
The dried samples (DCP and CDG) were treated with various concentrations of NaOH at levels of 2%, 4%, 6%, and 8% using a completely randomized design with 4 replications of each. After extraction, the residual DCP and CDG dietary fiber were dried in a hot air oven at 55°C to 60°C. Finally, the oven dried extracted dietary fiber was powdered to a particle size of 1 mm. Both extracted dietary fibers were analyzed for their chemical composition and determined by FTIR.
The DCP and CDG treated with NaOH linearly or quadratically or cubically (p< 0.05) increased the total dietary fiber (TDF) and insoluble fiber (IDF). The optimal conditions for extracting dietary fiber from DCP and CDG were under treatment with 6% and 4% NaOH, respectively, as these conditions yielded the highest TDF and IDF contents. These results were associated with the FTIR spectra integration for a semi-quantitative analysis, which obtained the highest cellulose content in dietary fiber extracted from DCP and CDG with 6% and 4% NaOH solution, respectively. The principal component analysis illustrated clear separation of spectral distribution in cassava pulp extracted dietary fiber (DFCP) and cassava distiller's dried grains extracted dietary fiber (DFCDG) when treated with 6% and 4% NaOH, respectively.
The optimal conditions for the extraction of dietary fiber from DCP and CDG were treatment with 6% and 4% NaOH solution, respectively. In addition, FTIR spectroscopy proved itself to be a powerful tool for fiber identification.
本研究旨在探究在不同氢氧化钠浓度下从木薯干浆（DCP）和木薯酒糟（CDG）中提取膳食纤维的条件，并利用傅里叶变换红外光谱（FTIR）测定膳食纤维成分。
采用完全随机设计，将干燥样品（DCP和CDG）分别用2%、4%、6%和8%的不同浓度氢氧化钠处理，每种处理重复4次。提取后，将残留的DCP和CDG膳食纤维在55°C至60°C的热风烘箱中干燥。最后，将烘干的提取膳食纤维粉碎至粒径为1mm。对两种提取的膳食纤维进行化学成分分析并通过FTIR测定。
用氢氧化钠处理的DCP和CDG使总膳食纤维（TDF）和不溶性纤维（IDF）呈线性、二次或三次增加（p<0.05）。从DCP和CDG中提取膳食纤维的最佳条件分别是用6%和4%的氢氧化钠处理，因为这些条件下TDF和IDF含量最高。这些结果与FTIR光谱积分进行半定量分析相关，该分析分别在从用6%和4%氢氧化钠溶液提取的DCP和CDG膳食纤维中获得了最高的纤维素含量。主成分分析表明，分别用6%和4%氢氧化钠处理时，木薯干浆提取的膳食纤维（DFCP）和木薯酒糟提取的膳食纤维（DFCDG）的光谱分布有明显分离。
从DCP和CDG中提取膳食纤维的最佳条件分别是用6%和4%的氢氧化钠溶液处理。此外，FTIR光谱证明是一种强大的纤维鉴定工具。
