Fortes Bruno Duarte Alves, de Carvalho Mello Heloisa Helena, Café Marcos Barcellos, Arnhold Emmanuel, Stringhini José Henrique
Federal Institute of Education, Science and Technology Goiano, Iporá, 76200-000, Brazil.
Department of Animal Science, Federal University of Goiás, Goiás, 74690-900, Brazil.
Anim Biosci. 2022 Jul;35(7):1030-1038. doi: 10.5713/ab.21.0416. Epub 2022 Jan 5.
Two experiments were conducted to evaluate the effect of enzyme complex (EC) on the standardized ileal digestibility (SID) of amino acids (AA) in corn gluten meal (60%) (CGM), soy protein concentrate (SPC), dried bovine plasma (DBP), and poultry offal meal (POM). Experiments I and II were conducted with broilers in the pre-starter (1 to 7 days of age) and starter (1 to 21 days of age) phases, respectively.
The treatments consisted of a protein-free diet (PFD) containing feedstuffs either supplemented with EC (xylanase, amylase, and protease) or not. In Experiment I, a total of 360 one-day-old male Cobb-500 broiler chicks were randomly housed in 45 pens, resulting in five replicates with eight birds each, totalizing eight treatments and one PFD group. In Experiment II a total of 270 one-day-old male Cobb-500 broiler chicks were randomly housed in 45 pens, resulting in five replicates with six birds each, totalizing eight treatments and one PFD group. The PFD groups were used to assess the endogenous AA losses. The birds were slaughtered to collect the ileal content.
In the pre-starter phase, the SID of arginine, branched chain-aminoacids, glycine, serine, aspartate, and glutamic acid increased with EC addition. The EC improved the SID of arginine and glutamic acid of CGM; the SID of valine and cystine of SPC; the SID of leucine, glycine, and aspartate of POM and the SID of isoleucine of DBP. In the starter phase, the SID of isoleucine, phenylalanine and glycine increased in EC-supplemented diets. The EC improved the SID of isoleucine of DBP; the SID of phenylalanine of CGM and POM. The SID of AA of SPC was not influenced by the EC.
The addition of an EC to broiler pre-starter and starter diets is efficient in increasing the SID of AA on SPC, POM, and DBP.
进行两项试验以评估复合酶（EC）对玉米蛋白粉（60%）（CGM）、大豆浓缩蛋白（SPC）、干血浆蛋白粉（DBP）和禽内脏粉（POM）中氨基酸（AA）标准回肠消化率（SID）的影响。试验I和试验II分别选用1至7日龄的雏鸡前期肉鸡和1至21日龄的雏鸡期肉鸡。
试验处理包括一种不含蛋白质的基础日粮（PFD），该日粮所含饲料原料分别添加或不添加EC（木聚糖酶、淀粉酶和蛋白酶）。在试验I中，总共360只1日龄雄性科宝500肉鸡雏鸡被随机饲养在45个鸡栏中，每组重复5次，每组8只鸡，共有8种处理和1个PFD组。在试验II中，总共270只1日龄雄性科宝500肉鸡雏鸡被随机饲养在45个鸡栏中，每组重复5次，每组6只鸡，共有8种处理和1个PFD组。PFD组用于评估内源氨基酸损失。鸡只屠宰后收集回肠内容物。
在雏鸡前期阶段，添加EC后精氨酸、支链氨基酸、甘氨酸、丝氨酸、天冬氨酸和谷氨酸的SID增加。EC提高了CGM中精氨酸和谷氨酸的SID；SPC中缬氨酸和胱氨酸的SID；POM中亮氨酸、甘氨酸和天冬氨酸的SID以及DBP中异亮氨酸的SID。在雏鸡期阶段，添加EC的日粮中异亮氨酸、苯丙氨酸和甘氨酸的SID增加。EC提高了DBP中异亮氨酸的SID；CGM和POM中苯丙氨酸的SID。SPC中氨基酸的SID不受EC影响。
在肉鸡雏鸡前期和雏鸡期日粮中添加EC可有效提高SPC、POM和DBP中氨基酸的SID。
