Gully Kay, Celton Jean-Marc, Degrave Alexandre, Pelletier Sandra, Brisset Marie-Noelle, Bucher Etienne
IRHS (Institut de Recherche en Horticulture et Semences), UMR 1345, INRA, Agrocampus-Ouest, Université d'Angers, SFR 4207 QuaSaV, Beaucouzé F-49071, France.
Epigenomes. 2019 Jan 14;3(1):3. doi: 10.3390/epigenomes3010003.
Under natural growth conditions, plants experience various and repetitive biotic and abiotic stresses. Salicylic acid (SA) is a key phytohormone involved in the response to biotic challenges. Application of synthetic SA analogues can efficiently prime defense responses, and leads to improved pathogen resistance. Because SA analogues can result in long-term priming and memory, we identified genes for which expression was affected by the SA analogue and explored the role of DNA methylation in this memorization process. We show that treatments with an SA analogue can lead to long-term transcriptional memory of particular genes in . We found that subsequent challenging of such plants with a bacterial elicitor reverted this transcriptional memory, bringing their expression back to the original pre-treatment level. We also made very similar observations in apple (), suggesting that this expression pattern is highly conserved in plants. Finally, we found a potential role for DNA methylation in the observed transcriptional memory behavior. We show that plants defective in DNA methylation pathways displayed a different memory behavior. Our work improves our understanding of the role of transcriptional memory in priming, and has important implication concerning the application of SA analogues in agricultural settings.
在自然生长条件下，植物会经历各种反复出现的生物和非生物胁迫。水杨酸（SA）是一种参与应对生物挑战的关键植物激素。合成SA类似物的应用可以有效地引发防御反应，并提高病原体抗性。由于SA类似物可导致长期引发和记忆，我们鉴定了那些表达受SA类似物影响的基因，并探索了DNA甲基化在这一记忆过程中的作用。我们表明，用SA类似物处理可导致特定基因在[具体植物名称未给出]中的长期转录记忆。我们发现，随后用细菌激发子对这些植物进行刺激会逆转这种转录记忆，使其表达恢复到处理前的原始水平。我们在苹果（[具体植物名称未给出]）中也观察到了非常相似的现象，这表明这种表达模式在植物中高度保守。最后，我们发现DNA甲基化在观察到的转录记忆行为中具有潜在作用。我们表明，DNA甲基化途径有缺陷的植物表现出不同的记忆行为。我们的工作增进了我们对转录记忆在引发过程中作用的理解，并且对于SA类似物在农业环境中的应用具有重要意义。
