磷酸化3-脱氧甘露糖辛酸作为流感嗜血杆菌脂多糖成分的鉴定。
Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide.
作者信息
Zamze S E, Ferguson M A, Moxon E R, Dwek R A, Rademacher T W
机构信息
Department of Paediatrics, University of Oxford, John Radcliffe Hospital, Headington, U.K.
出版信息
Biochem J. 1987 Jul 15;245(2):583-7. doi: 10.1042/bj2450583.
Abstract
A phosphorylated 3-deoxy-manno-octulosonic acid (KDO) was released from the lipopolysaccharide (LPS) of the deep rough mutant (Rb+169) of Haemophilus influenzae by acid hydrolysis. Both phosphorylated and dephosphorylated KDO, produced by treatment with alkaline phosphatase, were identified by gas chromatography-mass spectrometry after trimethylsilylation. This technique provides a rapid and reliable method for the identification of phosphorylated KDO in LPS.
摘要
通过酸水解从流感嗜血杆菌深度粗糙突变体（Rb + 169）的脂多糖（LPS）中释放出磷酸化的3-脱氧甘露糖辛酮酸（KDO）。用碱性磷酸酶处理产生的磷酸化和去磷酸化的KDO，在三甲基硅烷化后通过气相色谱 - 质谱法进行鉴定。该技术为鉴定LPS中的磷酸化KDO提供了一种快速可靠的方法。
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