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高丛蓝莓的完整叶绿体基因组()。

The complete chloroplast genome of highbush blueberry ().

作者信息

Miao Xiao-Rong, Chen Qiu-Xing, Niu Jun-Qi, Guo Yi-Peng

机构信息

Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Yulin Normal University, Yulin, China.

出版信息

Mitochondrial DNA B Resour. 2021 Dec 20;7(1):87-88. doi: 10.1080/23802359.2021.2009384. eCollection 2022.

DOI:10.1080/23802359.2021.2009384
PMID:34993320
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8725824/
Abstract

Highbush blueberry is a small berry fruit tree belonging to the family Ericaceae and genus , which fruit has high nutritional value. High-throughput sequencing technology was applied in this study to sequence and assemble the whole chloroplast genome of the southern highbush blueberry variety sharpblue. The results of the study showed that the circular genome of sharpblue is 170,737 bp in length, and the GC content of the genome was 36.8%. The complete chloroplast genome of sharpblue has consisted of two inverted repeat regions (IRs), a large single-copy region (LSC, 31, 076 bp), and a small single-copy region (SSC, 3, 044 bp). The chloroplast genome contained a total of 144 functional genes, including 100 mRNA genes, eight rRNA genes, and 36 tRNA genes. In addition, and were clustered into one group in this phylogenetic analysis which indicated that they have a close evolutionary relationship. The findings of this investigation are a significant reference source for the phylogeny and evolutionary origin of the Ericaceae family.

摘要

高丛蓝莓是一种属于杜鹃花科越橘属的小型浆果果树,其果实具有很高的营养价值。本研究应用高通量测序技术对南方高丛蓝莓品种夏普蓝的叶绿体全基因组进行测序和组装。研究结果表明,夏普蓝的环状基因组长度为170,737 bp,基因组的GC含量为36.8%。夏普蓝完整的叶绿体基因组由两个反向重复区域(IRs)、一个大单拷贝区域(LSC,31,076 bp)和一个小单拷贝区域(SSC,3,044 bp)组成。叶绿体基因组共包含144个功能基因,包括100个mRNA基因、8个rRNA基因和36个tRNA基因。此外,在该系统发育分析中,[此处原文缺失相关内容]和[此处原文缺失相关内容]被聚类为一组,这表明它们具有密切的进化关系。本研究结果是杜鹃花科系统发育和进化起源的重要参考来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b38/8725824/4b5efeef9c5d/TMDN_A_2009384_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b38/8725824/4b5efeef9c5d/TMDN_A_2009384_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b38/8725824/4b5efeef9c5d/TMDN_A_2009384_F0001_B.jpg

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本文引用的文献

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Brief Bioinform. 2019 Jul 19;20(4):1160-1166. doi: 10.1093/bib/bbx108.
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MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.
“组学”对质体行为促进类胡萝卜素积累的见解。
Front Plant Sci. 2022 Oct 6;13:1001756. doi: 10.3389/fpls.2022.1001756. eCollection 2022.
MEGA7:适用于更大数据集的分子进化遗传学分析版本7.0
Mol Biol Evol. 2016 Jul;33(7):1870-4. doi: 10.1093/molbev/msw054. Epub 2016 Mar 22.
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SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing.SPAdes:一种新的基因组组装算法及其在单细胞测序中的应用
J Comput Biol. 2012 May;19(5):455-77. doi: 10.1089/cmb.2012.0021. Epub 2012 Apr 16.
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NGS QC Toolkit: a toolkit for quality control of next generation sequencing data.NGS QC 工具包:下一代测序数据质量控制工具包。
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