巨噬细胞在登革病毒向B淋巴细胞呈递抗原过程中的必要作用。
Obligatory role of macrophages in dengue virus antigen presentation to B lymphocytes.
作者信息
Rizvi N, Chaturvedi U C, Mathur A
机构信息
Postgraduate Department of Microbiology, K.G. Medical College, Lucknow, India.
出版信息
Immunology. 1989 May;67(1):38-43.
The study was undertaken to investigate the role of dengue type 2 virus (DV)-infected mouse peritoneal macrophages (M phi) in presentation of the DV antigen to B lymphocytes as shown by counting virus-specific IgM antibody plaque-forming cells (PFC). It was observed that heat-killed or glutaraldehyde-fixed M phi did not present the antigen. Pretreatment of M phi with the lysosomotropic compounds ammonium chloride and chloroquine inhibited the antigen presentation. Depletion of M phi from the spleen cell cultures abrogated the immune response to DV. The tryptic-digested DV antigen could stimulate immune responses in B-lymphocyte enriched (depleted of M phi and T cells) spleen cell cultures, and the digested antigen could be presented by glutaraldehyde-fixed M phi. Pretreatment of M phi with a trypsin inhibitor abrogated antigen presentation. The findings thus show that even for presentation to B cells the DV antigen must be processed by M phi by a trypsin-like protease.
本研究旨在通过计数病毒特异性IgM抗体空斑形成细胞（PFC），来调查2型登革病毒（DV）感染的小鼠腹腔巨噬细胞（M phi）在将DV抗原呈递给B淋巴细胞过程中的作用。观察到热灭活或经戊二醛固定的M phi不呈递抗原。用溶酶体亲和性化合物氯化铵和氯喹预处理M phi可抑制抗原呈递。从脾细胞培养物中去除M phi消除了对DV的免疫反应。经胰蛋白酶消化的DV抗原可在富含B淋巴细胞（去除了M phi和T细胞）的脾细胞培养物中刺激免疫反应，且消化后的抗原可由经戊二醛固定的M phi呈递。用胰蛋白酶抑制剂预处理M phi可消除抗原呈递。因此，这些发现表明，即使对于呈递给B细胞，DV抗原也必须由M phi通过一种类胰蛋白酶进行处理。
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