Chaturvedi U C, Nagar R, Mathur A
J Gen Virol. 1983 Nov;64 (Pt 11):2399-407. doi: 10.1099/0022-1317-64-11-2399.
Fc-receptor-mediated attachment and ingestion of opsonized sheep erythrocytes (EA) by the macrophages of spleen and peritoneal cavity were studied during dengue virus type 2 (DV) infection of Swiss albino mice. Following intracerebral inoculation, virus antigen could be demonstrated by immunofluorescence in the splenic macrophages from day 4 and in peritoneal macrophages from day 5 post-infection, with a higher number of positive cells discernible on the 7th and 8th days. The virus could be isolated from spleen tissue from day 5. The total number of cells was markedly reduced from day 4 onwards both in the spleen and peritoneal cavity. A loss in the capacity to attach and ingest EA was noticed, the lowest values of attachment index (AI) and phagocytic index (PI) being reached on day 4. At later periods the AI values increased markedly but continued to be significantly less than those in uninfected control mice. The PI values continued to be lower throughout. The dichotomy between the Fc-mediated attachment and ingestion may be a mechanism for prevention of virus infection of macrophages.
在瑞士白化小鼠感染2型登革病毒（DV）期间，研究了脾脏和腹腔巨噬细胞通过Fc受体介导对调理过的绵羊红细胞（EA）的附着和摄取情况。脑内接种后，感染后第4天可通过免疫荧光在脾脏巨噬细胞中检测到病毒抗原，第5天在腹腔巨噬细胞中检测到，在第7天和第8天可观察到更多的阳性细胞。从第5天起可从脾脏组织中分离出病毒。从第4天起，脾脏和腹腔中的细胞总数均显著减少。观察到附着和摄取EA的能力下降，附着指数（AI）和吞噬指数（PI）的最低值在第4天达到。在后期，AI值显著增加，但仍持续显著低于未感染对照小鼠。PI值始终较低。Fc介导的附着和摄取之间的二分法可能是巨噬细胞预防病毒感染的一种机制。
