Shenzhen Key Laboratory of Special Functional Materials & Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen, 518060, China.
Shenzhen Key Laboratory of Special Functional Materials & Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen, 518060, China.
Biosens Bioelectron. 2022 Apr 1;201:113958. doi: 10.1016/j.bios.2021.113958. Epub 2022 Jan 2.
Heart-type fatty acid binding protein (H-FABP) has been regarded as a promising biomarker for early diagnosis of acute myocardial infarction (AMI). Developing fast and reliable method for H-FABP detection is still highly desirable but challenging. Herein, an ascorbic acid (AA)-mediated organic photoelectrochemical transistor (OPECT) sensing strategy was reported for the detection of H-FABP in phosphate buffer saline (PBS) solution and human serum. A primary antibody/H-FABP/secondary antibody-Au NPs-alkaline phosphatase (ALP) sandwich immunorecognition structure was constructed. The modified ALP could catalytically convert ascorbic acid-2-phosphate to AA, which was then analyzed by OPECT. As a result, the AA-mediated OPECT sensing strategy realized highly sensitive detection of H-FABP with a detection limit of 3.23 × 10 g/mL which is two orders of magnitude lower than that of PEC method. Under optimal experimental conditions, H-FABP concentration could be obtained in ∼90 min. Importantly, the analysis of H-FABP was resistant to the interference from immunoglobulin G, bovine serum albumin, cysteine, AA and human serum. The proposed AA-mediated OPECT sensing strategy provides a simple, fast, and accurate way for H-FABP detection in AMI suspected patients.
心脏型脂肪酸结合蛋白（H-FABP）已被认为是急性心肌梗死（AMI）早期诊断的有前途的生物标志物。开发快速可靠的 H-FABP 检测方法仍然是非常需要的，但具有挑战性。本文报道了一种抗坏血酸（AA）介导的有机光电晶体管（OPECT）传感策略，用于检测磷酸盐缓冲盐水（PBS）溶液和人血清中的 H-FABP。构建了初级抗体/H-FABP/二级抗体-Au NPs-碱性磷酸酶（ALP）三明治免疫识别结构。修饰后的 ALP 可催化将抗坏血酸-2-磷酸转化为 AA，然后通过 OPECT 进行分析。结果，AA 介导的 OPECT 传感策略实现了 H-FABP 的高灵敏度检测，检测限为 3.23×10 -3 g/mL，比 PEC 方法低两个数量级。在最佳实验条件下，约 90 min 内即可获得 H-FABP 浓度。重要的是，H-FABP 的分析不受免疫球蛋白 G、牛血清白蛋白、半胱氨酸、AA 和人血清的干扰。提出的 AA 介导的 OPECT 传感策略为 AMI 疑似患者的 H-FABP 检测提供了一种简单、快速、准确的方法。
