Punnonen J, Eskola J
Department of Medical Microbiology, Turku University, Finland.
Acta Pathol Microbiol Immunol Scand C. 1987 Aug;95(4):167-72. doi: 10.1111/j.1699-0463.1987.tb00026.x.
Highly purified normal human tonsillar B lymphocytes were cultured with recombinant IL 2 (rIL 2). The responses were minimal when the cells were cultured with rIL 2 alone. Dose-dependent proliferation and differentiation into immunoglobulin-secreting cells were found when the cells were simultaneously activated with Staphylococcus aureus Cowan I (SAC). T cells were not found to proliferate in these cultures. B-cell responses were not augmented when 16% T cells were added to the B-cell population. In contrast, B-cell proliferation and Ig secretion were significantly enhanced by adding 0.5% T cells when cultured in the presence of pokeweed mitogen (PWM). Moreover, B-cell stimulation by rIL 2 was detected at very low cell densities. It is concluded that IL 2 directly affects the function of activated B cells.
用重组白细胞介素2（rIL-2）培养高度纯化的正常人扁桃体B淋巴细胞。当细胞单独用rIL-2培养时反应极小。当细胞同时用金黄色葡萄球菌考恩I株（SAC）激活时，发现有剂量依赖性增殖并分化为免疫球蛋白分泌细胞。在这些培养物中未发现T细胞增殖。当向B细胞群体中加入16%的T细胞时，B细胞反应未增强。相反，在有商陆丝裂原（PWM）存在的情况下培养时，加入0.5%的T细胞可显著增强B细胞增殖和Ig分泌。此外，在非常低的细胞密度下也检测到rIL-2对B细胞的刺激作用。得出的结论是，IL-2直接影响活化B细胞的功能。
