Wrigley D M, Choi Y S
Cell Immunol. 1983 May;78(1):130-43. doi: 10.1016/0008-8749(83)90266-6.
Staphylococcus aureus Cowan I strain (SAC) activates human peripheral blood B lymphocytes to proliferate but does not induce secretion of immunoglobulin (Ig) unless pokeweed mitogen (PWM)-activated T cells are added. Using a Percoll gradient separation method, we show that a B-cell subpopulation isolated at 50/60% Percoll interface is the target for SAC stimulation. After SAC activation, small, dense B cells become larger, less-dense B cells which proliferate. These activated B blasts differentiate into Ig-secreting cells when cultured with PWM-activated T cells. The culture supernatant of PWM-activated T cells is capable of supporting proliferation and Ig secretion of the activated B blasts but not of resting small B cells. This result suggests that the culture supernatant contains B-cell growth factors.
金黄色葡萄球菌考恩I株（SAC）可激活人外周血B淋巴细胞增殖，但除非加入商陆有丝分裂原（PWM）激活的T细胞，否则不会诱导免疫球蛋白（Ig）分泌。使用Percoll梯度分离法，我们发现分离于50/60%Percoll界面的B细胞亚群是SAC刺激的靶细胞。SAC激活后，小而致密的B细胞会变成更大、密度更低且增殖的B细胞。当与PWM激活的T细胞共培养时，这些活化的B母细胞会分化为Ig分泌细胞。PWM激活的T细胞的培养上清液能够支持活化B母细胞的增殖和Ig分泌，但不能支持静止小B细胞的增殖和Ig分泌。这一结果表明培养上清液中含有B细胞生长因子。
