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漆酶:各种类型和应用。

Laccase: Various types and applications.

机构信息

Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

Biotechnol Appl Biochem. 2022 Dec;69(6):2658-2672. doi: 10.1002/bab.2313. Epub 2022 Jan 18.

Abstract

Laccase belongs to the polyphenol oxidase family and is very important in removing environmental pollutants due to its structural and functional properties. Recently, the ability of laccase to oxidize phenolic and nonphenolic substances has been considered by many researchers. This enzyme's application scope includes a broad range of chemical processes and industrial usages, such as bioremediation, nanobiotechnology, woodworking industries, bleaching of paper pulp, dyeing in the textile industry, biotechnological uses in food industries, biorefining, detoxification from wastewater, production of organic matter from phenolic and amine substrates, and biofuels. Although filamentous fungi produce large amounts of laccase, high-yield industrial-scale production of laccase is still faced with many problems. At present, researchers are trying to increase the efficiency and productivity and reduce the final price of laccase by finding suitable microorganisms and improving the process of production and purification of laccase. This article reviews the introduction of laccase, its properties, production processes, and the effect of various factors on the enzyme's stability and activity, and some of its applications in various industries.

摘要

漆酶属于多酚氧化酶家族,由于其结构和功能特性,在去除环境污染物方面非常重要。最近,许多研究人员都考虑到了漆酶氧化酚类和非酚类物质的能力。这种酶的应用范围包括广泛的化学过程和工业用途,如生物修复、纳米生物技术、木材加工业、纸浆漂白、纺织工业中的染色、食品工业中的生物技术用途、生物炼制、从废水中解毒、从酚类和胺类底物中生产有机物以及生物燃料。尽管丝状真菌会产生大量的漆酶,但高产的工业规模生产漆酶仍面临许多问题。目前,研究人员正在通过寻找合适的微生物以及改进漆酶的生产和纯化工艺,来提高效率和生产力并降低最终的漆酶价格。本文综述了漆酶的介绍、性质、生产工艺以及各种因素对酶稳定性和活性的影响,并介绍了其在各个行业中的一些应用。

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