Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, Republic of Korea.
Solus Advanced Materials Co., Ltd., Yongin 16858, Republic of Korea.
J Dairy Sci. 2022 Mar;105(3):1929-1939. doi: 10.3168/jds.2021-21192. Epub 2022 Jan 5.
Ceramide-containing phospholipids improve skin hydration and barrier function and are ideal for use in skin care products. In this study, we evaluated the photoprotective effect of milk phospholipids on the skin condition of UVB-irradiated hairless mice. Skin parameters were assessed following oral administration of milk phospholipids. The UVB irradiation induced photoaging in mice. The animals were divided into 5 groups: a control group (oral administration of saline with no UBV irradiation), UVB group (oral administration of saline with UVB irradiation), and 3 UVB irradiation groups receiving the milk phospholipids at 3 different concentrations of oral administration, 50 mg/kg (ML group), 100 mg/kg (MM group), and 150 mg/kg (MH group), for 8 wk. An increase in skin hydration and transepidermal water loss were improved in the 150 mg/kg of milk phospholipid-administered group. Hematoxylin and eosin staining revealed a decrease in epidermal thickness in the milk phospholipid-administered groups (50, 100, and 150 mg/kg of body weight). In particular, the 100 and 150 mg/kg groups showed significant changes in the area, length, and depth of the wrinkles compared with the UVB group. Moreover, the gene expression of matrix metalloproteins was attenuated, and that of proinflammatory cytokines, especially tumor necrosis factor-α, was significantly reduced in the milk phospholipid-administered groups than in the UVB group. The reduced ceramide and increased sphingosine-1-phosphate levels in the skin tissue due to UVB exposure were restored to levels similar to those of the control group following milk phospholipid administration. These results were confirmed to be due to the downregulation of protein expression of nuclear factor kappa-B (NF-κB) and phosphorylated IκB-α (inhibitor of κB α). Collectively, oral administration of milk phospholipids improves skin health through a synergistic effect on photoprotective activity.
含神经酰胺的磷脂可改善皮肤的水合作用和屏障功能，是理想的护肤品成分。本研究评估了牛奶磷脂对 UVB 照射无毛小鼠皮肤状况的光保护作用。口服牛奶磷脂后评估皮肤参数。UVB 照射诱导小鼠光老化。将动物分为 5 组：对照组（口服生理盐水，不进行 UVB 照射）、UVB 组（口服生理盐水，进行 UVB 照射）和 3 个口服不同浓度牛奶磷脂的 UVB 照射组，浓度分别为 50mg/kg（ML 组）、100mg/kg（MM 组）和 150mg/kg（MH 组），连续 8 周。150mg/kg 牛奶磷脂组皮肤水合度和经表皮水分丢失增加。H&E 染色显示牛奶磷脂给药组表皮厚度降低（50、100 和 150mg/kg 体重）。特别是 100 和 150mg/kg 组与 UVB 组相比，皱纹的面积、长度和深度均有显著变化。此外，与 UVB 组相比，牛奶磷脂给药组基质金属蛋白酶的基因表达减弱，促炎细胞因子，特别是肿瘤坏死因子-α的基因表达显著降低。UVB 暴露导致皮肤组织中神经酰胺减少和鞘氨醇-1-磷酸增加，经牛奶磷脂给药后恢复至对照组水平。这些结果被确认为由于核因子 kappa-B（NF-κB）和磷酸化 IκB-α（κB α 抑制剂）的蛋白表达下调所致。综上所述，口服牛奶磷脂通过对光保护活性的协同作用改善皮肤健康。
