Lauerma A I, Visa K, Pekonen M, Förström L, Reitamo S
Department of Dermatology, Helsinki University Central Hospital, Finland.
Arch Dermatol Res. 1987;279(6):379-84. doi: 10.1007/BF00412623.
The phenotypes of the infiltrating cells in 13 patients with delayed hypersensitivity to topical glucocorticosteroids (GCS) were studied from sequential biopsies of positive epicutaneous test reactions by using the avidin-biotin-complex (ABC) technique. Monoclonal antibodies were used to identify the cells with the following phenotypes: T3, T4/T4a, T6, T8, T9, T11, M1, Ia1 (HLA-DR), interleukin-2 receptor/T26a, and dendritic reticular cell. The cellular kinetics of GCS hypersensitivity reactions were compared with delayed hypersensitivity reactions caused by allergens not related to GCS. In both GCS and non-GCS reactions the epidermal dendritic T6+ cells were more numerous than dendritic Ia1+ cells. There was a decrease in the number of both cell types during these reactions; in GCS reactions the decrease in the number of T6+ cells was seen later than in non-GCS reactions. Ia1+ keratinocytes were seen at sites near dermal infiltrates. Compared with the non-GCS delayed hypersensitivity reaction, there were fewer pan T (T11+/T3+) in the GCS reaction. The relative numbers of M1+ monocytes and the T4/T8 ratio were substantially lower in the latter; these findings can be explained as a GCS effect which modulates the delayed type hypersensitivity reaction.
采用抗生物素蛋白-生物素复合物（ABC）技术，通过对13例对局部糖皮质激素（GCS）迟发型超敏反应患者的阳性皮肤试验反应进行连续活检，研究了浸润细胞的表型。使用单克隆抗体来识别具有以下表型的细胞：T3、T4/T4a、T6、T8、T9、T11、M1、Ia1（HLA-DR）、白细胞介素-2受体/T26a和树突状网状细胞。将GCS超敏反应的细胞动力学与由与GCS无关的变应原引起的迟发型超敏反应进行了比较。在GCS和非GCS反应中，表皮树突状T6+细胞均比树突状Ia1+细胞多。在这些反应过程中，两种细胞类型的数量均减少；在GCS反应中，T6+细胞数量的减少比非GCS反应中出现得晚。在真皮浸润附近的部位可见Ia1+角质形成细胞。与非GCS迟发型超敏反应相比，GCS反应中的全T细胞（T11+/T3+）较少。后者中M1+单核细胞的相对数量和T4/T8比值显著较低；这些发现可以解释为GCS对迟发型超敏反应的调节作用。
