Song Wei, Wang Ya-Chao, Chen Huang, Li Xiangzhu, Zhou Lingxiao, Min Changjun, Zhu Siwei, Yuan Xiaocong
Nanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology, Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen, China.
Department of Neurosurgery, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China.
J Biophotonics. 2022 May;15(5):e202100329. doi: 10.1002/jbio.202100329. Epub 2022 Jan 20.
The ability to unveil molecular specificities of endogenous nonfluorescent chromophores of ultraviolet photoacoustic imaging technology enables label-free histology imaging of tissue specimens. In this work, we exploit ultraviolet photoacoustic microscopy for identifying human glioma xenograft of mouse brain ex vivo. Intrinsically excellent imaging contrast of cell nucleus at ultraviolet photoacoustic illumination along with good spatial resolution allows for discerning the brain glioma of freshly-harvested thick brain slices, which circumvents laborious time-consuming preparations of the tissue specimens including micrometer-thick slicing and H&E staining that are prerequisites in standard histology analysis. The identification of tumor margins and quantitative analysis of tumor areas is implemented, representing good agreement with the standard H&E-stained observations. Quantitative ultraviolet photoacoustic microscopy can access fast pathological assessment to the brain tissues, and thus potentially facilitates intraoperative brain tumor resection to precisely remove all cancerous cells and preserve healthy tissue for maintaining its essential function.
紫外光声成像技术能够揭示内源性非荧光发色团的分子特异性，从而实现组织标本的无标记组织学成像。在这项工作中，我们利用紫外光声显微镜对小鼠脑内的人胶质瘤异种移植进行离体鉴定。紫外光声照明下细胞核具有出色的成像对比度，同时具备良好的空间分辨率，这使得我们能够辨别新鲜采集的厚脑切片中的脑胶质瘤，避免了标准组织学分析中繁琐且耗时的组织标本制备过程，如微米级切片和苏木精-伊红染色。我们实现了肿瘤边缘的识别和肿瘤面积的定量分析，与标准苏木精-伊红染色观察结果高度吻合。定量紫外光声显微镜能够对脑组织进行快速病理评估，从而有可能在术中辅助脑肿瘤切除，精确切除所有癌细胞并保留健康组织以维持其基本功能。
