Department of Ultrasound, General Hospital of People's Liberation Army, Beijing, China; Key Laboratory of Molecular Imaging of Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, Beijing, China.
CAS Engineering Laboratory for Nanozyme, Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Nanomedicine. 2020 Jul;27:102204. doi: 10.1016/j.nano.2020.102204. Epub 2020 Apr 12.
The vexing difficulty in distinguishing glioma from normal tissues is a major obstacle to prognosis. In an attempt to solve this problem, we used a joint strategy that combined targeted-cancer stem cells nanoparticles with precise photoacoustic and fluorescence navigation. We showed that traditional magnetic resonance imaging (MRI) did not represent the true morphology of tumors. Targeted nanoparticles specifically accumulated in the tumor area. Glioma was precisely revealed at the cellular level. Tumors could be non-invasively detected through the intact skull by fluorescence molecular imaging (FMI) and photoacoustic tomography (PAT). Moreover, PAT can be used to excise deep gliomas. Histological correlation confirmed that FMI imaging accurately delineated scattered tumor cells. The combination of optical PAT and FMI navigation fulfilled the promise of precise visual imaging in glioma detection and resection. This detection method was deeper and more intuitive than the current intraoperative pathology.
区分神经胶质瘤与正常组织的困难是影响预后的主要障碍。为了解决这个问题，我们采用了联合策略，将靶向肿瘤干细胞的纳米颗粒与精确的光声和荧光导航相结合。我们发现传统的磁共振成像（MRI）不能代表肿瘤的真实形态。靶向纳米颗粒特异性地聚集在肿瘤区域。在细胞水平上准确地揭示了神经胶质瘤。通过荧光分子成像（FMI）和光声断层扫描（PAT）可以无创地检测到完整颅骨下的肿瘤。此外，PAT 可用于切除深部神经胶质瘤。组织学相关性证实，FMI 成像准确地描绘了散在的肿瘤细胞。光学 PAT 和 FMI 导航的结合实现了神经胶质瘤检测和切除中精确可视化成像的承诺。这种检测方法比目前的术中病理检查更深、更直观。
