Kerdsin Anusak, Hatrongjit Rujirat, Wongsurawat Thidathip, Jenjaroenpun Piroon, Chopjitt Peechanika, Boueroy Parichart, Fittipaldi Nahuel, Zheng Han, Gottschalk Marcelo
Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.
Department of General Sciences, Faculty of Science and Engineering, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.
Front Microbiol. 2021 Dec 23;12:812436. doi: 10.3389/fmicb.2021.812436. eCollection 2021.
is a zoonotic pathogen that causes invasive infections in humans and pigs. Although serotype 2 is prevalent among patient and swine infections, other serotypes are occasionally detected in humans. Of these, serotype 24 clonal complex (CC) 221/234 are recognized as emerging clones of human infection. Genomic exploration of three serotype 24 CC221/234 strains revealed antimicrobial resistance genes, pathotyping, virulence-associated gene (VAG) profiles, minimum core genome (MCG) typing, and comparison of the genomes. Based on these analyzes, all three serotype 24 strains were MCG7-3 and should be classified in the intermediate/weakly virulent (I/WV) group. All selected serotype 24 strains were susceptible to several antibiotics including β-lactam, fluoroquinolone, and chloramphenicol. Resistance to tetracycline, macrolide, and clindamycin was observed and attributed to the genes and . Genomic comparison revealed the strains S12X, LSS66, LS0L, LS0E, 92-4,172, and IMT40201 that had phylogenetic affinity with serotype 24 CC221/234. Analysis of 80 virulence-associated genes (VAG) showed that all three serotype 24 strains lacked 24 genes consisting of adhesin P, , permease , and Eleven specific sequences were identified in the 3 serotype 24 genomes that differed from the genomes of the representative strains of epidemic (E; SC84), highly virulent (HV; P1/7), I/WV (89-1,591), and avirulent (T15 and 05HAS68).
是一种人畜共患病原体，可导致人类和猪发生侵袭性感染。虽然2型血清型在患者和猪感染中普遍存在，但其他血清型偶尔也会在人类中被检测到。其中，24型血清型克隆复合体（CC）221/234被认为是人类感染的新兴克隆。对三株24型血清型CC221/234菌株进行基因组探索，揭示了抗菌抗性基因、致病型、毒力相关基因（VAG）谱、最小核心基因组（MCG）分型以及基因组比较。基于这些分析，所有三株24型血清型菌株均为MCG7-3，应归类于中等/弱毒力（I/WV）组。所有选定的24型血清型菌株对包括β-内酰胺、氟喹诺酮和氯霉素在内的几种抗生素敏感。观察到对四环素、大环内酯和克林霉素的抗性，并归因于基因 和 。基因组比较揭示了与24型血清型CC221/234具有系统发育亲和力的菌株S12X、LSS66、LS0L、LS0E、92-4、172和IMT40201。对80个毒力相关基因（VAG）的分析表明，所有三株24型血清型菌株均缺少由粘附素P、 、通透酶 和 组成的24个基因。在三株24型血清型基因组中鉴定出11个特定序列，这些序列与流行株（E；SC84）、高毒力株（HV；P1/7）、I/WV株（89-1,591）和无毒力株（T15和05HAS68）的代表性菌株基因组不同。
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