Chaiden Chadaporn, Jaresitthikunchai Janthima, Phaonakrop Narumon, Roytrakul Sittiruk, Kerdsin Anusak, Nuanualsuwan Suphachai
Department of Veterinary Public Health, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Functional Proteomics Technology Laboratory, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology for Development Agency, Pathum Thani 12120, Thailand.
Animals (Basel). 2021 Aug 24;11(9):2480. doi: 10.3390/ani11092480.
() is a zoonotic pathogen causing severe streptococcal disease worldwide. infections in pigs and humans are frequently associated with the virulent serotype 2 (SS2). Though various virulence factors of have been proposed, most of them were not essentially accounted for in the experimental infections. In the present study, we compared the peptidomes of highly virulent SS2 and SS14 in humans, the swine causative serotypes SS7 and SS9, and the rarely reported serotypes SS25 and SS27, and they were cultured in a specified culture medium containing whole blood to simulate their natural host environment. LC-MS/MS could identify 22 unique peptides expressed in the six serotypes. Under the host-simulated environment, peptides from the ABC-type phosphate transport system (SSU05_1106) and 30S ribosomal protein S2 (B) were detected in the peptidome of virulent SS2 and SS14. Therefore, we suggest that these two proteins or their derived peptides might be involved in the survival of when simulated with a blood environment.
（某病原体）是一种人畜共患病原体，在全球范围内引发严重的链球菌病。猪和人类的感染通常与强毒力的2型血清型（SS2）有关。尽管已提出了该病原体的多种毒力因子，但在实验感染中大多未对其进行本质上的阐释。在本研究中，我们比较了人类中高毒力SS2和SS14、猪致病血清型SS7和SS9以及罕见报道的血清型SS25和SS27的肽组，并且将它们在含有全血的特定培养基中培养以模拟其自然宿主环境。液相色谱-串联质谱（LC-MS/MS）能够鉴定出在这六种血清型中表达的22种独特肽段。在宿主模拟环境下，在强毒力SS2和SS14的肽组中检测到来自ABC型磷酸盐转运系统（SSU05_1106）和30S核糖体蛋白S2（B）的肽段。因此，我们认为这两种蛋白质或其衍生肽段在模拟血液环境时可能参与了该病原体的存活。
