Obert David Peter, Killing David, Happe Tom, Altunkaya Alp, Schneider Gerhard, Kreuzer Matthias, Fenzl Thomas
Department of Anesthesiology and Intensive Care Medicine, Technical University of Munich, School of Medicine, Munich, Germany.
MethodsX. 2021 Jul 21;8:101466. doi: 10.1016/j.mex.2021.101466. eCollection 2021.
Establishing a long-lasting, functioning venous access in a non-anesthetized mouse is very challenging at least. Since we needed a reliable venous access to titrate intravenous anesthetics, we refined and combined previously described methods. The tunneling of the catheter from the cranial to the pectoral wound, the fixation of the catheter in the external jugular vein with two sutures, and a tissue adhesive allowed us to combine this method with the implantation of intracranial recording electrodes. With this approach we neither have to restrain the animal causing excessive stress nor do we need an additional anesthetic, interfering with the effects of the intravenous anesthetic. This approach can help to establish a greater understanding of the concept of consciousness by identifying the neural circuits which mediate the effect of intravenous anesthetics. In addition - due to the flexible design of the recording electrode array - our approach can also be applied to investigate further neuroscientific hypotheses.•Establishment of a reliable chronical venous access for the application in freely behaving mice.•The jugular venous access can be combined with all kinds of neurobiological recording and application designs.•The design of the venous access allows chronic combinations with telemetric and tether-bound systems.
至少在未麻醉的小鼠身上建立持久、有效的静脉通路极具挑战性。由于我们需要可靠的静脉通路来滴定静脉麻醉剂，我们改进并结合了先前描述的方法。将导管从颅骨创口向胸部创口进行隧道式植入，用两根缝线将导管固定于颈外静脉，并使用组织粘合剂，这使我们能够将该方法与颅内记录电极的植入相结合。通过这种方法，我们既无需束缚动物而造成过度应激，也无需额外使用麻醉剂，以免干扰静脉麻醉剂的效果。这种方法有助于通过识别介导静脉麻醉剂作用的神经回路，加深对意识概念的理解。此外，由于记录电极阵列的灵活设计，我们的方法还可用于进一步研究神经科学假说。
•为自由活动的小鼠建立可靠的长期静脉通路以用于实验。
•颈静脉通路可与各种神经生物学记录及应用设计相结合。
•静脉通路的设计允许与遥测和系绳系统进行长期组合。
