Homolak Jan, Kodvanj Ivan, Babic Perhoc Ana, Virag Davor, Knezovic Ana, Osmanovic Barilar Jelena, Riederer Peter, Salkovic-Petrisic Melita
Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia.
Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia.
MethodsX. 2021 Dec 22;9:101611. doi: 10.1016/j.mex.2021.101611. eCollection 2022.
We propose a rapid, simple, and robust method for measurement of the reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations.•NRP is a rapid, cost-effective, and simple method for reductive capacity assessment•NRP is compatible with a high-throughput screening of solid and liquid biological samples•HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis.
我们提出了一种快速、简单且可靠的方法，用于基于高锰酸钾还原并随后将二氧化锰沉淀捕获在硝酸纤维素膜上来测量液体和固体生物样品的还原能力。此外，我们讨论了硝酸纤维素氧化还原电位滴定法（NRP）如何用于生物样品的高通量分析，并介绍了组织NRP，它是用于详细分析保留解剖关系的组织中还原能力空间分布的改良方法。•NRP是一种用于评估还原能力的快速、经济高效且简单的方法•NRP与固体和液体生物样品的高通量筛选兼容•组织NRP利用被动扩散切片印迹法进行还原能力空间分析。
