Xu Jianqing, Ren Zhihong, Cao Kangli, Li Xianping, Yang Jing, Luo Xuelian, Zhu Lingyan, Wang Xiangwei, Ding Longfei, Liang Junrong, Jin Dong, Yuan Tingting, Li Lianfeng, Xu Jianguo
Zhongshan Hospital, Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Front Nutr. 2021 Dec 22;8:789242. doi: 10.3389/fnut.2021.789242. eCollection 2021.
Boosting and prolonging SARS-CoV-2 vaccine-elicited immunity is paramount for containing the COVID-19 pandemic, which wanes substantially within months after vaccination. Here we demonstrate that the unique strain of probiotic GUANKE (LPG) could promote SARS-CoV-2-specific immune responses in both effective and memory phases through enhancing interferon signaling and suppressing apoptotic and inflammatory pathways. Interestingly, oral LPG administration promoted SARS-CoV-2 neutralization antibodies even 6 months after immunization. Furthermore, when LPG was given immediately after SARS-CoV-2 vaccine inoculation, specific neutralization antibodies could be boosted >8-fold in bronchoalveolar lavage (BAL) and >2-fold in sera, T-cell responses were persistent and stable for a prolonged period both in BAL and the spleen. Transcriptional analyses showed that oral application of LPG mobilized immune responses in the mucosal and systemic compartments; in particular, gut-spleen and gut-lung immune axes were observed. These results suggest that LPG could be applied in combination with SARS-CoV-2 vaccines to boost and prolong both the effective and memory immune responses in mucosal and systemic compartments, thereby improving the efficacy of SARS-CoV-2 vaccination.
增强和延长新冠病毒疫苗诱导的免疫力对于控制新冠疫情至关重要，因为疫苗接种后几个月内免疫力会大幅下降。在此，我们证明了益生菌冠科（LPG）的独特菌株可通过增强干扰素信号传导以及抑制凋亡和炎症途径，在有效阶段和记忆阶段促进新冠病毒特异性免疫反应。有趣的是，口服LPG即使在免疫6个月后仍能促进新冠病毒中和抗体的产生。此外，在接种新冠病毒疫苗后立即给予LPG，支气管肺泡灌洗（BAL）中的特异性中和抗体可增强8倍以上，血清中可增强2倍以上，BAL和脾脏中的T细胞反应在较长时间内持续且稳定。转录分析表明，口服LPG可调动黏膜和全身区室的免疫反应；特别是，观察到了肠-脾和肠-肺免疫轴。这些结果表明，LPG可与新冠病毒疫苗联合应用，以增强和延长黏膜和全身区室的有效免疫反应和记忆免疫反应，从而提高新冠病毒疫苗接种的效果。
