Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki City, 852-8523, Japan.
Section of Pharmacognosy, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan.
J Nat Med. 2022 Mar;76(2):402-409. doi: 10.1007/s11418-021-01596-w. Epub 2022 Jan 10.
The emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in 2019 has led to a global health crisis. Mutations of the SARS-CoV-2 genome have impeded the development of effective therapeutics and vaccines against SARS-CoV-2. Natural products are important for discovering therapeutics to treat the 2019 coronavirus disease (COVID-19). In the present study, we investigated the antiviral activity of herbal drug extracts from Polygala Root, Areca, and Quercus Bark and natural compounds derived from herbal drug such as baicalin and glabridin, with IC values of 9.5 µg/ml, 1.2 µg/ml, 5.4 µg/ml, 8.8 µM, and 2.5 µM, respectively, against SARS CoV-2 infection in vitro. Certain herbal drug extracts and natural compounds were found to inhibit viral RNA levels and infectious titers of SARS-CoV-2 in a dose-dependent manner. Furthermore, viral protein analyses showed that herbal drug extracts and natural compounds effectively inhibited SARS-CoV-2 in the various entry treatments. Our study revealed that three herbal drugs are good candidates for further in vivo and clinical studies.
2019 年严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 大流行的出现导致了全球卫生危机。SARS-CoV-2 基因组的突变阻碍了针对 SARS-CoV-2 的有效治疗方法和疫苗的开发。天然产物对于发现治疗 2019 年冠状病毒病 (COVID-19) 的疗法非常重要。在本研究中，我们研究了从远志根、槟榔和栎树皮中提取的草药药物提取物以及源自草药的天然化合物，如黄芩苷和甘草素，对 SARS-CoV-2 体外感染的抗病毒活性，其 IC 值分别为 9.5µg/ml、1.2µg/ml、5.4µg/ml、8.8µM 和 2.5µM。某些草药药物提取物和天然化合物被发现以剂量依赖性方式抑制 SARS-CoV-2 的病毒 RNA 水平和感染滴度。此外，病毒蛋白分析表明，草药药物提取物和天然化合物有效地抑制了 SARS-CoV-2 的各种进入处理。我们的研究表明，三种草药是进一步进行体内和临床研究的良好候选药物。
