Molecular Sciences and Engineering Laboratory, Amity Institute of Click Chemistry Research and Studies, Amity University Uttar Pradesh, Noida 201301, India.
Environmental Biochemistry & Molecular Biology Laboratory, Department of Biochemistry, University College of Medical Sciences & GTB Hospital, University of Delhi, Delhi 110095, India.
ACS Appl Bio Mater. 2021 Aug 16;4(8):6304-6315. doi: 10.1021/acsabm.1c00570. Epub 2021 Jul 13.
Concerning the climate crisis, energy disaster, and greenhouse effects, microalgae have paved the way for consideration as a biofuel feed material. The advent of polymeric materials with unique architecture at nanoscale, in combination with microalgae, has given direction for the bioeconomic yield of highly valued compounds, essentially lipid. Herein, we discuss the paramount significance of exotic hydrogel matrix (HM) with efficient violet light absorption, far-red emission, CO-adsorbing capability and catalyst-free condition that could increase the photosynthesis activity, alleviating the microalgal growth for the effective augmentation of lipid, protein, and chlorophyll. The intrinsic morphological and structural features of HM were revealed by a suite of characterizations that confirm its hollow tubular architecture. Fluorescence intensity measurement confirmed the electron transfer from HM to , accelerating the photosynthetic rate for the improved production of lipids (98%), proteins (60%), and chlorophyll a (121%), compared to untreated control cells. Moreover, by visualizing the Nile red (NR) fluorescence response from /HM cells, a high lipid content was observed with a larger cell size (14.6 μm) compared to control cells (8.7 μm). Fatty acid methyl esters (FAMEs), obtained from /HM, were noted with a large-scale volume of C16:C18 fatty acids (>80%). We, therefore, envisage that HM plays a significant role in enhancing the generation of lipids and proteins from . These outcomes assure a qualitative transit in the bioenergy domain.
关于气候危机、能源灾难和温室效应，微藻作为生物燃料的原料已经引起了人们的关注。具有独特纳米级结构的聚合材料的出现，与微藻相结合，为高价值化合物（本质上是脂质）的生物经济产量指明了方向。在这里，我们讨论了具有高效紫光吸收、远红光发射、CO 吸附能力和无催化剂条件的外来水凝胶基质 (HM) 的至关重要性，这可以提高光合作用活性，缓解微藻生长，从而有效增加脂质、蛋白质和叶绿素。通过一系列的表征揭示了 HM 的内在形态和结构特征，证实了其空心管状结构。荧光强度测量证实了电子从 HM 转移到 ，从而加速了光合作用速率，使脂质（增加 98%）、蛋白质（增加 60%）和叶绿素 a（增加 121%）的产量得到提高，与未经处理的 对照细胞相比。此外，通过观察 /HM 细胞中的尼罗红 (NR) 荧光响应，可以观察到与对照细胞（8.7 μm）相比，HM 具有更高的脂质含量和更大的细胞尺寸（14.6 μm）。从 /HM 获得的脂肪酸甲酯 (FAMEs) 具有大量的 C16:C18 脂肪酸（>80%）。因此，我们认为 HM 在增强 产生脂质和蛋白质方面发挥了重要作用。这些结果确保了生物能源领域的定性转变。
