Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.
Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea.
ACS Appl Bio Mater. 2021 Aug 16;4(8):6046-6055. doi: 10.1021/acsabm.1c00346. Epub 2021 Jul 13.
Bone graft materials have been mainly developed based on inorganic materials, including calcium phosphate. However, these graft materials usually act as osteoconductive rather than osteoinductive scaffolds. To improve bone reconstruction, a combination of several materials has been proposed. However, there are still no alternatives that can completely replace the existing animal-derived bone graft materials. In this work, a marine-inspired biomineral complex was suggested as a potential bone graft material. The proposed biosilicified coccolithophore-derived coccoliths using bioengineered mussel adhesive proteins show osteopromotive ability through the synergistic effects of osteoconductivity from calcium carbonate and osteoinductivity from silica. Its possibility of use as a bone substitute was determined by evaluating the in vitro osteogenic behaviors of multipotent mesenchymal stem cells and in vivo bone regeneration in a rat calvarial defect model. Therefore, the marine-inspired biomineral complex developed in this study could be successfully used for bone tissue engineering.
骨移植材料主要是基于无机材料开发的，包括磷酸钙。然而，这些移植材料通常作为骨传导而不是骨诱导支架。为了改善骨重建，已经提出了几种材料的组合。但是，仍然没有可以完全替代现有的动物源性骨移植材料的替代品。在这项工作中，提出了一种受海洋启发的生物矿化复合物作为潜在的骨移植材料。所提出的使用生物工程贻贝黏附蛋白的生物矿化颗石藻衍生颗石具有通过碳酸钙的骨传导性和硅的骨诱导性的协同作用表现出促骨生成能力。通过评估多能间充质干细胞的体外成骨行为和大鼠颅骨缺损模型中的体内骨再生，确定了其作为骨替代物的可能性。因此，本研究开发的受海洋启发的生物矿化复合物可成功用于骨组织工程。
