Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 37673, South Korea.
Department of Dentistry, Anam Hospital, Korea University Medical Center, Seoul, 02841, South Korea.
Adv Healthc Mater. 2016 Dec;5(24):3191-3202. doi: 10.1002/adhm.201601169. Epub 2016 Nov 29.
Xenogenic bone substitutes are commonly used during orthopedic reconstructive procedures to assist bone regeneration. However, huge amounts of blood accompanied with massive bone loss usually increase the difficulty of placing the xenograft into the bony defect. Additionally, the lack of an organic matrix leads to a decrease in the mechanical strength of the bone-grafted site. For effective bone grafting, this study aims at developing a mussel adhesion-employed bone graft binder with great blood-resistance and enhanced mechanical properties. The distinguishing water (or blood) resistance of the binder originates from sandcastle worm-inspired complex coacervation using negatively charged hyaluronic acid (HA) and a positively charged recombinant mussel adhesive protein (rMAP) containing tyrosine residues. The rMAP/HA coacervate stabilizes the agglomerated bone graft in the presence of blood. Moreover, the rMAP/HA composite binder enhances the mechanical and hemostatic properties of the bone graft agglomerate. These outstanding features improve the osteoconductivity of the agglomerate and subsequently promote in vivo bone regeneration. Thus, the blood-resistant coacervated mussel protein glue is a promising binding material for effective bone grafting and can be successfully expanded to general bone tissue engineering.
异种骨替代品在骨科重建手术中常用于辅助骨再生。然而，大量的血液伴随着大量的骨质流失通常会增加异种移植物放置到骨缺损部位的难度。此外，缺乏有机基质会导致骨移植部位的机械强度降低。为了实现有效的骨移植，本研究旨在开发一种具有良好抗血液能力和增强机械性能的贻贝黏附性骨移植结合剂。该结合剂的独特抗水（或血液）能力源于受沙堡蠕虫启发的复杂共凝聚作用，使用带负电荷的透明质酸 (HA) 和带正电荷的含有酪氨酸残基的重组贻贝黏附蛋白 (rMAP)。在血液存在的情况下，rMAP/HA 共沉淀物稳定了聚集的骨移植物。此外，rMAP/HA 复合结合剂增强了骨移植物聚集物的机械和止血性能。这些优异的特性提高了聚集物的骨诱导性，从而促进体内骨再生。因此，抗血液共凝聚贻贝蛋白胶是一种有前途的有效骨移植结合材料，可成功扩展到一般的骨组织工程中。
