State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, P. R. China.
ACS Appl Bio Mater. 2021 Aug 16;4(8):6361-6370. doi: 10.1021/acsabm.1c00583. Epub 2021 Aug 2.
Pathogenic bacterial contamination in diverse environments seriously threatens human health. One of the most valuable approaches is to effectively combine sensitive detection with efficient sterilization to achieve source control of pathogens. Here, we constructed a nanoplatform of BiS@MnO@Van with targeting, photothermal, and oxidase properties for the detection and on-demand inactivation of bacteria. The BiS@MnO@Van nanorods (NRs) can be trapped on the surface of Gram-positive bacteria such as , forming a complex of BiS@MnO@Van/. After being centrifuged, the suspension of BiS@MnO@Van NRs can catalyze the non-fluorescent Amplex Red (AR) into a fluorescent substrate and fluorescent Scopoletin (SC) into a non-fluorescent substrate. Thus, a ratiometric fluorescent sensor was constructed for the sensitive detection of bacteria with the fluorescent intensity ratio (SC/AR) as a readout, which improves anti-interference capability and can be used in real sample detection. The detection limit reaches as low as 6.0 CFU/mL. Meanwhile, the sediment contains BiS@MnO@Van/ where the bacteria can be effectively inactivated, thanks to the excellent photothermal property of BiS@MnO@Van NRs under near-infrared irradiation. The antibacterial efficiency reaches as high as 99.1%. The investigation provides an effective way for sensitive detection and highly efficient killing of pathogenic bacteria with a universal platform.
不同环境中的致病细菌污染严重威胁着人类健康。其中最有价值的方法之一是将灵敏检测与高效杀菌有效结合，实现病原体的源头控制。在这里，我们构建了一种具有靶向、光热和氧化酶特性的 BiS@MnO@Van 纳米平台，用于细菌的检测和按需灭活。BiS@MnO@Van 纳米棒（NRs）可以捕获革兰氏阳性菌（如 ）的表面，形成 BiS@MnO@Van/复合物。离心后，BiS@MnO@Van NRs 的悬浮液可以催化非荧光性 Amplex Red（AR）转化为荧光底物和非荧光性 Scopoletin（SC）。因此，构建了一种比率荧光传感器，用于以荧光强度比（SC/AR）作为读出值的细菌灵敏检测，这提高了抗干扰能力，并可用于实际样品检测。检测限低至 6.0 CFU/mL。同时，沉淀物中含有 BiS@MnO@Van/，由于 BiS@MnO@Van NRs 在近红外照射下具有优异的光热性能，其中的细菌可以被有效灭活。抗菌效率高达 99.1%。该研究为具有通用平台的致病细菌灵敏检测和高效杀灭提供了一种有效方法。
