Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland.
Laboratory for Biointerfaces, Department Materials Meet Life, Empa Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland.
ACS Appl Bio Mater. 2021 Jun 21;4(6):5295-5303. doi: 10.1021/acsabm.1c00415. Epub 2021 May 12.
Surface-associated microbial infections and contaminations are a major challenge in various fields including the food and health sectors. This study demonstrates the design of antimicrobial coatings based on the self-assembly of the food-grade amphiphilic lipid glycerol monooleate with the human cathelicidin-derived antimicrobial peptide LL-37. Structural properties of the coating and their alterations with composition were studied using advanced experimental methods including synchrotron grazing-incidence small-angle X-ray scattering and ellipsometry. The integration of the LL-37 and its potential release from the nanostructured films into the surrounding solution was characterized with confocal Raman microscopy. Additional biological evaluation studies with clinically relevant bacterial strains, namely, (Gram-negative) and (Gram-positive), were performed to investigate the antimicrobial activity of the coatings. Significant killing activity of the coating was found against both bacterial strains. The presented findings contribute to the fundamental understanding of lipid-peptide self-assembly on the surface and may open up a promising strategy for designing simple, sustainable antimicrobial coatings for medical and food applications.
表面相关的微生物感染和污染是包括食品和健康部门在内的各个领域的主要挑战。本研究展示了基于食品级两亲性脂质甘油单油酸酯与源自人防御素的抗菌肽 LL-37 的自组装的抗菌涂层的设计。使用包括同步辐射掠入射小角 X 射线散射和椭圆光度法在内的先进实验方法研究了涂层的结构特性及其随组成的变化。使用共聚焦拉曼显微镜对 LL-37 的整合及其从纳米结构薄膜向周围溶液中的潜在释放进行了表征。还对临床相关细菌菌株 （革兰氏阴性）和 （革兰氏阳性）进行了额外的生物学评估研究，以研究涂层的抗菌活性。发现该涂层对两种细菌菌株均具有显著的杀伤活性。本研究结果有助于深入了解表面上的脂质-肽自组装，并可能为设计用于医疗和食品应用的简单、可持续抗菌涂层开辟一条有前景的策略。
