Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100149, China.
Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100149, China.
Genomics Proteomics Bioinformatics. 2021 Oct;19(5):689-706. doi: 10.1016/j.gpb.2021.08.012. Epub 2022 Jan 8.
In the past decade, relative proteomic quantification using isobaric labeling technology has developed into a key tool for comparing the expression of proteins in biological samples. Although its multiplexing capacity and flexibility make this a valuable technology for addressing various biological questions, its quantitative accuracy and precision still pose significant challenges to the reliability of its quantification results. Here, we give a detailed overview of the different kinds of isobaric mass tags and the advantages and disadvantages of the isobaric labeling method. We also discuss which precautions should be taken at each step of the isobaric labeling workflow, to obtain reliable quantification results in large-scale quantitative proteomics experiments. In the last section, we discuss the broad applications of the isobaric labeling technology in biological and clinical studies, with an emphasis on thermal proteome profiling and proteogenomics.
在过去的十年中，使用等重标记技术的相对蛋白质组定量已发展成为比较生物样本中蛋白质表达的重要工具。尽管其多重检测能力和灵活性使其成为解决各种生物学问题的有价值的技术，但它的定量准确性和精密度仍然对其定量结果的可靠性提出了重大挑战。在这里，我们详细介绍了不同种类的等重质量标签，以及等重标记方法的优缺点。我们还讨论了在等重标记工作流程的每个步骤中应该采取哪些预防措施，以在大规模定量蛋白质组学实验中获得可靠的定量结果。在最后一节中，我们讨论了等重标记技术在生物学和临床研究中的广泛应用，重点介绍了热蛋白质组谱分析和蛋白质基因组学。
