Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
Zhejiang Guowei Technology Co., Ltd, Zhuji, Zhejiang 311800, China.
Poult Sci. 2022 Mar;101(3):101641. doi: 10.1016/j.psj.2021.101641. Epub 2021 Dec 1.
The abuse of antibiotics for agricultural purposes has been under scrutiny. Therefore, there is an urgent need to find antibiotic substitutes in animal production. The effects of chlorogenic acid, β-D-Glucan, astragalus flavone, CpG-DNA, and chicken IgG on spleen antioxidant capacity, apoptosis, and the immune response in Shaoxing ducklings were investigated in this study. The ducklings treated with β-D-Glucan, astragalus flavone, CpG-DNA, and chicken IgG showed significant reduction in catalase and superoxide dismutase activities. The five immunopotentiators facilitated caspase 3 expression and reduced Bcl2 expression in the spleen. Compared to the control group, the protein level of COX2 was significantly upregulated in the chlorogenic acid, CpG-DNA, and chicken IgG groups. The protein level of iNOS expression was significantly improved in all immunopotentiator groups, except for the astragalus flavone group. The five immunopotentiators induced IL-1β, IFN-α, IFN-β, TNF-α, RIG-I, TLR3, and TLR7 gene expression. In summary, chlorogenic acid, β-D-Glucan, astragalus flavone, CpG-DNA, and chicken IgG, as immunopotentiators, improved the innate immune response in the ducklings, which not only provides a new avenue for the development of efficient approaches to prevent pathogen infections, but also offers an alternative to antibiotics in animal production.
抗生素在农业中的滥用一直受到关注。因此，在动物生产中寻找抗生素替代品已成为当务之急。本研究探讨了绿原酸、β-D-葡聚糖、黄芪黄酮、CpG-DNA 和鸡 IgG 对绍兴鸭脾脏抗氧化能力、细胞凋亡和免疫反应的影响。β-D-葡聚糖、黄芪黄酮、CpG-DNA 和鸡 IgG 处理的鸭脾脏中过氧化氢酶和超氧化物歧化酶活性显著降低。五种免疫增强剂促进了 caspase 3 的表达，降低了脾脏中 Bcl2 的表达。与对照组相比，绿原酸、CpG-DNA 和鸡 IgG 组 COX2 蛋白水平显著上调。除黄芪黄酮组外，所有免疫增强剂组的 iNOS 表达蛋白水平均显著提高。五种免疫增强剂诱导了鸭 IL-1β、IFN-α、IFN-β、TNF-α、RIG-I、TLR3 和 TLR7 基因的表达。综上所述，绿原酸、β-D-葡聚糖、黄芪黄酮、CpG-DNA 和鸡 IgG 作为免疫增强剂，提高了鸭的固有免疫反应，不仅为开发预防病原体感染的有效方法提供了新途径，而且为动物生产中的抗生素替代提供了可能。
