Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, Poland.
Int J Mol Sci. 2021 Dec 25;23(1):214. doi: 10.3390/ijms23010214.
The ZCR6 strain, isolated from the rhizosphere of growing in soil co-contaminated with hydrocarbons and heavy metals, was investigated for its plant growth promotion, hydrocarbon degradation, and heavy metal resistance. In vitro bioassays confirmed all of the abovementioned properties. ZCR6 was able to produce indole acetic acid (IAA), siderophores, and ammonia, solubilized Ca(PO), and showed surface active properties and activity of cellulase and very high activity of 1-aminocyclopropane-1-carboxylic acid deaminase (297 nmol α-ketobutyrate mg h). The strain degraded petroleum hydrocarbons (76.52% of the initial hydrocarbon content was degraded) and was resistant to Cd, Zn, and Cu (minimal inhibitory concentrations reached 5, 15, and 10 mM metal, respectively). The genome of the ZCR6 strain consisted of 5,507,067 bp, and a total of 5055 genes were annotated, of which 4943 were protein-coding sequences. Annotation revealed the presence of genes associated with nitrogen fixation, phosphate solubilization, sulfur metabolism, siderophore biosynthesis and uptake, synthesis of IAA, ethylene modulation, heavy metal resistance, exopolysaccharide biosynthesis, and organic compound degradation. Complete characteristics of the ZCR6 strain showed its potential multiway properties for enhancing the phytoremediation of co-contaminated soils. To our knowledge, this is the first analysis of the biotechnological potential of the species .
从生长在石油烃和重金属共存污染土壤中的根际中分离到的 ZCR6 菌株，因其具有促进植物生长、降解烃类化合物和抗重金属的特性而受到研究。体外生物测定证实了上述所有特性。ZCR6 能够产生吲哚乙酸（IAA）、铁载体和氨，溶解 Ca（PO），并具有表面活性、纤维素酶活性和 1-氨基环丙烷-1-羧酸脱氨酶（297 nmol α-酮丁酸 mg h）的极高活性。该菌株能够降解石油烃（初始烃含量的 76.52%被降解），并对 Cd、Zn 和 Cu 具有抗性（最小抑制浓度分别达到 5、15 和 10 mM 金属）。ZCR6 菌株的基因组由 5,507,067 bp 组成，共注释了 5055 个基因，其中 4943 个是蛋白质编码序列。注释揭示了与固氮、磷酸盐溶解、硫代谢、铁载体生物合成和摄取、IAA 合成、乙烯调节、重金属抗性、胞外多糖生物合成和有机化合物降解相关的基因的存在。ZCR6 菌株的完整特征表明其具有增强共污染土壤植物修复的多方面潜力。据我们所知，这是对该物种生物技术潜力的首次分析。
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