Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, University of Basel, 4031 Basel, Switzerland.
Genomic Research Laboratory, Geneva University Hospitals, University of Geneva, 1206 Geneva, Switzerland.
Int J Mol Sci. 2021 Dec 29;23(1):381. doi: 10.3390/ijms23010381.
We present the case of a 72-year-old female patient with acute contained rupture of a biological composite graft, 21 months after replacement of the aortic valve and the ascending aorta due to an aortic dissection. Auramine-rhodamine staining of intraoperative biopsies showed acid-fast bacilli, but classical culture and molecular methods failed to identify any organism. Metagenomic analysis indicated infection with , which was confirmed by target-specific qPCR. The complexity of the sample required a customized bioinformatics pipeline, including cleaning steps to remove sequences of human, bovine ad pig origin. Our study underlines the importance of multiple testing to increase the likelihood of pathogen identification in highly complex samples.
我们报告了一例 72 岁女性患者的病例，该患者在因主动脉夹层而行主动脉瓣和升主动脉置换 21 个月后，发生生物复合移植物急性局限性破裂。术中活检的金胺-罗丹明染色显示抗酸杆菌，但经典培养和分子方法均未能鉴定出任何病原体。宏基因组分析提示感染了 ，靶向特异性 qPCR 证实了这一点。由于样本的复杂性，需要定制生物信息学分析流程，包括清除步骤以去除人、牛和猪来源的序列。我们的研究强调了进行多次检测以增加高度复杂样本中病原体鉴定可能性的重要性。
