Innovative Research Laboratory for Wound Healing, Health Sciences Department, Medical School, Università del Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy.
Department of Sciences and Technological Innovation, Università del Piemonte Orientale, Via T. Michel 11, 15121 Alessandria, Italy.
Int J Mol Sci. 2022 Jan 4;23(1):539. doi: 10.3390/ijms23010539.
In recent years, bioprinting has attracted much attention as a potential tool for generating complex 3D biological constructs capable of mimicking the native tissue microenvironment and promoting physiologically relevant cell-cell and cell-matrix interactions. The aim of the present study was to develop a crosslinked 3D printable hydrogel based on biocompatible natural polymers, gelatin and xanthan gum at different percentages to be used both as a scaffold for cell growth and as a wound dressing. The CellInk Inkredible 3D printer was used for the 3D printing of hydrogels, and a glutaraldehyde solution was tested for the crosslinking process. We were able to obtain two kinds of printable hydrogels with different porosity, swelling and degradation time. Subsequently, the printed hydrogels were characterized from the point of view of biocompatibility. Our results showed that gelatin/xanthan-gum bioprinted hydrogels were biocompatible materials, as they allowed both human keratinocyte and fibroblast in vitro growth for 14 days. These two bioprintable hydrogels could be also used as a helpful dressing material.
近年来，生物打印作为一种潜在的工具，吸引了广泛的关注，它能够生成复杂的 3D 生物结构体，模拟天然组织微环境，并促进生理相关的细胞-细胞和细胞-基质相互作用。本研究旨在开发一种基于生物相容性天然聚合物（明胶和黄原胶）的交联 3D 可打印水凝胶，其不同比例可同时用作细胞生长的支架和伤口敷料。使用 CellInk Inkredible 3D 打印机进行水凝胶的 3D 打印，并测试了戊二醛溶液的交联过程。我们成功获得了两种具有不同孔隙率、溶胀性和降解时间的可打印水凝胶。随后，从生物相容性的角度对打印水凝胶进行了表征。我们的结果表明，明胶/黄原胶生物打印水凝胶是生物相容性材料，因为它们允许体外培养的人角质形成细胞和成纤维细胞生长 14 天。这两种可生物打印的水凝胶也可用作有用的敷料材料。
