Rudaya Elizaveta S, Kozyulina Polina Yu, Pavlova Olga A, Dolgikh Alexandra V, Ivanova Alexandra N, Dolgikh Elena A
All-Russia Research Institute for Agricultural Microbiology, Podbelsky chausse 3, Pushkin, 196608 St. Petersburg, Russia.
Komarov Botanical Institute RAS, Prof. Popov St., 2, 197376 St. Petersburg, Russia.
Plants (Basel). 2021 Dec 25;11(1):56. doi: 10.3390/plants11010056.
The IPD3/CYCLOPS transcription factor was shown to be involved in the regulation of nodule primordia development and subsequent stages of nodule differentiation. In contrast to early stages, the stages related to nodule differentiation remain less studied. Recently, we have shown that the accumulation of cytokinin at later stages may significantly impact nodule development. This conclusion was based on a comparative analysis of cytokinin localization between pea wild type and mutants. However, the role of cytokinin at these later stages of nodulation is still far from understood. To determine a set of genes involved in the regulation of later stages of nodule development connected with infection progress, intracellular accommodation, as well as plant tissue and bacteroid differentiation, the RNA-seq analysis of pea mutant SGEFix-2 () nodules impaired in these processes compared to wild type SGE nodules was performed. To verify cytokinin's influence on late nodule development stages, the comparative RNA-seq analysis of SGEFix-2 () mutant plants treated with cytokinin was also conducted. Findings suggest a significant role of cytokinin in the regulation of later stages of nodule development.
IPD3/CYCLOPS转录因子被证明参与根瘤原基发育及随后根瘤分化阶段的调控。与早期阶段相比，与根瘤分化相关的阶段研究较少。最近，我们发现后期细胞分裂素的积累可能会显著影响根瘤发育。这一结论是基于对豌豆野生型和突变体之间细胞分裂素定位的比较分析得出的。然而，细胞分裂素在根瘤形成后期阶段的作用仍远未明确。为了确定一组与根瘤发育后期调控相关的基因，这些后期阶段与感染进程、细胞内容纳以及植物组织和类菌体分化有关，我们对豌豆突变体SGEFix-2（）的根瘤进行了RNA测序分析，该突变体在这些过程中与野生型SGE根瘤相比存在缺陷。为了验证细胞分裂素对根瘤发育后期阶段的影响，我们还对用细胞分裂素处理的SGEFix-2（）突变体植株进行了比较RNA测序分析。研究结果表明细胞分裂素在根瘤发育后期调控中具有重要作用。
