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利用RNA测序技术在红三叶草中鉴定出与生物固氮效率相关的基因。

Genes Associated with Biological Nitrogen Fixation Efficiency Identified Using RNA Sequencing in Red Clover ( L.).

作者信息

Vlk David, Trněný Oldřich, Řepková Jana

机构信息

Department of Experimental Biology, Faculty of Sciences, Masaryk University, 611 37 Brno, Czech Republic.

Agricultural Research, Ltd., Zahradní 1, 664 41 Troubsko, Czech Republic.

出版信息

Life (Basel). 2022 Nov 25;12(12):1975. doi: 10.3390/life12121975.

DOI:10.3390/life12121975
PMID:36556339
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9785344/
Abstract

Commonly studied in the context of legume-rhizobia symbiosis, biological nitrogen fixation (BNF) is a key component of the nitrogen cycle in nature. Despite its potential in plant breeding and many years of research, information is still lacking as to the regulation of hundreds of genes connected with plant-bacteria interaction, nodulation, and nitrogen fixation. Here, we compared root nodule transcriptomes of red clover ( L.) genotypes with contrasting nitrogen fixation efficiency, and we found 491 differentially expressed genes (DEGs) between plants with high and low BNF efficiency. The annotation of genes expressed in nodules revealed more than 800 genes not yet experimentally confirmed. Among genes mediating nodule development, four nod-ule-specific cysteine-rich (NCR) peptides were confirmed in the nodule transcriptome. Gene duplication analyses revealed that genes originating from tandem and dispersed duplication are significantly over-represented among DEGs. Weighted correlation network analysis (WGCNA) organized expression profiles of the transcripts into 16 modules linked to the analyzed traits, such as nitrogen fixation efficiency or sample-specific modules. Overall, the results obtained broaden our knowledge about transcriptomic landscapes of red clover's root nodules and shift the phenotypic description of BNF efficiency on the level of gene expression in situ.

摘要

生物固氮（BNF）通常在豆科植物 - 根瘤菌共生的背景下进行研究，是自然界氮循环的关键组成部分。尽管其在植物育种方面具有潜力且经过多年研究，但对于数百个与植物 - 细菌相互作用、结瘤和固氮相关基因的调控，仍缺乏相关信息。在此，我们比较了固氮效率不同的红三叶草（L.）基因型的根瘤转录组，发现高固氮效率和低固氮效率的植物之间有491个差异表达基因（DEG）。对根瘤中表达基因的注释揭示了800多个尚未经实验证实的基因。在介导根瘤发育的基因中，在根瘤转录组中确认了四种根瘤特异性富含半胱氨酸（NCR）的肽。基因重复分析表明，源自串联重复和分散重复的基因在差异表达基因中显著富集。加权基因共表达网络分析（WGCNA）将转录本的表达谱组织成16个与分析性状相关的模块，如固氮效率或样本特异性模块。总体而言，所获得的结果拓宽了我们对红三叶草根瘤转录组景观的认识，并将生物固氮效率的表型描述转变为原位基因表达水平。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/3c3aa26c7d21/life-12-01975-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/330da6871333/life-12-01975-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/ab70f7ec1673/life-12-01975-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/8fbed882800d/life-12-01975-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/d427873c4111/life-12-01975-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/339d39b18892/life-12-01975-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/654296ad3537/life-12-01975-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/3c3aa26c7d21/life-12-01975-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/330da6871333/life-12-01975-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/ab70f7ec1673/life-12-01975-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/8fbed882800d/life-12-01975-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/d427873c4111/life-12-01975-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/339d39b18892/life-12-01975-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/654296ad3537/life-12-01975-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9876/9785344/3c3aa26c7d21/life-12-01975-g007.jpg

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Overexpression of Terpenoid Biosynthesis Genes From Garden Sage () Modulates Rhizobia Interaction and Nodulation in Soybean.
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