Choi Minsol, Sathasivam Ramaraj, Nguyen Bao Van, Park Nam Il, Woo Sun-Hee, Park Sang Un
Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea.
Department of Smart Agriculture Systems, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea.
Plants (Basel). 2021 Dec 28;11(1):90. doi: 10.3390/plants11010090.
Tartary buckwheat () is an important crop that belongs to the Polygonaceae family, whose roots have received considerable attention due to the presence of compounds with high nutritional and medicinal value. In this study, we aimed to develop an efficient protocol for the culture of adventitious (ARs) and hairy (HRs) roots on a half-strength Schenk and Hildebrandt (SH) medium containing different concentrations of the auxins, α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), and indole-3-acetic acid (IAA). The highest percentage of root induction (91.67%) was achieved with 0.5 mg/L IAA, whereas the greatest number of roots was found in 1 mg/L IAA. In contrast, 0.1 mg/L IBA returned the longest roots. As expected, HRs were obtained from in vitro leaf explants infected with R1000. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 11 phenolic pathway genes revealed that five genes (, , , , and ) were highly expressed in HRs, whereas only four (, , , and ), and three (, , and ) were recognized in the ARs and seedling roots (SRs), respectively. HPLC analysis of phenolic compounds in different root cultures showed that the majority of the phenolic compounds (both individual and total) were significantly accumulated in the HRs. Principal component analysis (PCA) identified differences among the three root types, whereby HRs were separated from ARs and SRs based on the amount of phenolic compounds present. Analysis of the metabolic pathway revealed that among the identified metabolites, the 3, 2, and 1 pathways were associated with flavonoid, flavone and flavonol, and phenylpropanoid biosynthesis, respectively. Hierarchical clustering analysis and the heat map showed that the different root cultures presented unique metabolites.
苦荞麦是蓼科的一种重要作物，因其根中含有具有高营养和药用价值的化合物而备受关注。在本研究中，我们旨在开发一种高效的方案，用于在含有不同浓度生长素α-萘乙酸（NAA）、吲哚-3-丁酸（IBA）和吲哚-3-乙酸（IAA）的1/2强度的 Schenk和Hildebrandt（SH）培养基上培养不定根（ARs）和毛状根（HRs）。用0.5mg/L IAA时，根诱导率最高（91.67%），而在1mg/L IAA时根的数量最多。相比之下，0.1mg/L IBA培养出的根最长。正如预期的那样，通过用发根农杆菌R1000感染离体叶片外植体获得了毛状根。对11个酚类途径基因的定量实时聚合酶链反应（qRT-PCR）分析表明，5个基因（[具体基因1]、[具体基因2]、[具体基因3]、[具体基因4]和[具体基因5]）在毛状根中高表达，而在不定根和幼苗根（SRs）中分别仅识别出4个（[具体基因6]、[具体基因7]、[具体基因8]和[具体基因9]）和3个（[具体基因10]、[具体基因11]和[具体基因12]）。对不同根培养物中酚类化合物的HPLC分析表明，大多数酚类化合物（单个和总量）在毛状根中显著积累。主成分分析（PCA）确定了三种根类型之间的差异，据此根据酚类化合物的含量将毛状根与不定根和幼苗根区分开来。代谢途径分析表明，在鉴定出的代谢物中，3、2和1途径分别与黄酮类、黄酮和黄酮醇以及苯丙烷类生物合成有关。层次聚类分析和热图显示，不同的根培养物呈现出独特的代谢物。
