Park Chanung, Sathasivam Ramaraj, Yeo Hyeon Ji, Park Young Jin, Kim Jae Kwang, Shin Su Young, Park Sang Un
Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea.
Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Republic of Korea.
ACS Omega. 2024 May 23;9(22):23761-23771. doi: 10.1021/acsomega.4c01735. eCollection 2024 Jun 4.
var. chinensis is a perennial monocot plant that is well known for its pharmaceutical and ornamental uses. This study aimed to understand the changes in the primary and secondary metabolites in different in vitro tissues of (callus, adventitious root, and shoot) using high-performance liquid chromatography and gas chromatography time-of-flight mass spectrometry. In addition, to optimize the most efficient in vitro culture methods for primary and secondary metabolite production, bulbs were used as explants and cultivated in Murashige and Skoog (MS) medium containing different hormones at various concentrations. In addition, the present study found suitable hormonal concentrations for callus, adventitious root, and shoot induction and analyzed the primary and secondary metabolites. The MS medium supplemented with 1.0 mg L dicamba, 3.0 mg L indole-3-butyric acid (IBA), and 3.0 mg L 6-benzylaminopurine (BAP) was the most efficient media for callus, adventitious root, and shoot induction in . The tissue induced in this medium was subjected to primary (amines, amino acids, organic acids, sugars, and sugar alcohols) and secondary metabolite (galantamine and phenolic acids) analysis. The shoots and roots showed the highest amounts of metabolites. This study showed that bulb in vitro culture can be an efficient micropropagation method for and the production of primary and secondary metabolites, offering implications for the mass production of primary and secondary metabolite compounds from tissues generated in vitro.
中华变种是一种多年生单子叶植物，以其药用和观赏用途而闻名。本研究旨在利用高效液相色谱法和气相色谱-飞行时间质谱法，了解（愈伤组织、不定根和芽）不同离体组织中初级和次级代谢产物的变化。此外，为了优化用于初级和次级代谢产物生产的最有效的离体培养方法，以鳞茎为外植体，在含有不同浓度不同激素的Murashige和Skoog（MS）培养基中培养。此外，本研究还发现了适合愈伤组织、不定根和芽诱导的激素浓度，并分析了初级和次级代谢产物。添加1.0 mg/L麦草畏、3.0 mg/L吲哚-3-丁酸（IBA）和3.0 mg/L 6-苄基腺嘌呤（BAP）的MS培养基是中华变种愈伤组织、不定根和芽诱导最有效的培养基。在该培养基中诱导的组织进行了初级（胺类、氨基酸、有机酸、糖类和糖醇类）和次级代谢产物（加兰他敏和酚酸）分析。芽和根中的代谢产物含量最高。本研究表明，鳞茎离体培养可以成为中华变种高效的微繁殖方法以及初级和次级代谢产物的生产方法，为从离体产生的中华变种组织中大量生产初级和次级代谢产物化合物提供了启示。
