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愈伤组织提取物对人包皮成纤维细胞蓝光损伤的作用及机制。

Effects and Mechanism of the Callus Culture Extract on Blue Light Damage in Human Foreskin Fibroblasts.

机构信息

College of Chemistry and Materials Engineering, Beijing Technology & Business University, Beijing 100048, China.

Acelbio (Chongqing) Biotechnology Co., Ltd., Chongqing 404100, China.

出版信息

Molecules. 2023 Feb 26;28(5):2172. doi: 10.3390/molecules28052172.

DOI:10.3390/molecules28052172
PMID:36903418
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10004670/
Abstract

is an important source of raw material for food, medicine, and modern cosmetics. The purpose of this study was to develop a new application for protection against blue light damage. To investigate the effects and mechanism of action of callus culture extract (LACCE) on blue light damage, a blue-light-induced human foreskin fibroblast damage model was established. The contents of collagen (COL-I), matrix metalloproteinase 1 (MMP-1), and opsin 3 (OPN3) were detected using enzyme-linked immunosorbent assays and Western blotting. The calcium influx and reactive oxygen species (ROS) levels were measured via flow cytometry and the results showed that the LACCE (10-15 mg/mL) promoted the production of COL-I, inhibited the secretion of MMP-1, OPN3, ROS and calcium influx, and may play a role in inhibiting the activation of blue light on the OPN3-calcium pathway. Thereafter, high-performance liquid chromatography and ultra-performance liquid chromatography-tandem mass spectrometry were used to quantitatively analyze the contents of nine active ingredients in the LACCE. The results indicated that LACCE has an anti-blue-light-damage effect and provides theoretical support for the development of new raw materials in the natural food, medicine, and skin care industries.

摘要

是食品、药品和现代化妆品的重要原料来源。本研究旨在开发一种新的应用，以防止蓝光损伤。为了研究愈伤组织培养提取物（LACCE）对蓝光损伤的作用及其作用机制，建立了蓝光诱导的人包皮成纤维细胞损伤模型。采用酶联免疫吸附试验和 Western blot 检测胶原（COL-I）、基质金属蛋白酶 1（MMP-1）和视蛋白 3（OPN3）的含量。通过流式细胞术测量钙内流和活性氧（ROS）水平，结果表明，LACCE（10-15mg/mL）促进 COL-I 的产生，抑制 MMP-1、OPN3、ROS 和钙内流的分泌，可能在抑制 OPN3-钙通路的蓝光激活方面发挥作用。随后，采用高效液相色谱法和超高效液相色谱-串联质谱法对 LACCE 中 9 种活性成分的含量进行定量分析。结果表明，LACCE 具有抗蓝光损伤作用，为天然食品、药品和皮肤护理行业新原料的开发提供了理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/ff1c2e629da1/molecules-28-02172-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/57dabe14d434/molecules-28-02172-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/27bef55b4b08/molecules-28-02172-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/ee35c11582d3/molecules-28-02172-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/57dd468f65de/molecules-28-02172-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/2d5ac496b76c/molecules-28-02172-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/6785a3385b3e/molecules-28-02172-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/67f0328db804/molecules-28-02172-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/0b97d8107503/molecules-28-02172-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/e4e7099faced/molecules-28-02172-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/ff1c2e629da1/molecules-28-02172-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/57dabe14d434/molecules-28-02172-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/27bef55b4b08/molecules-28-02172-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/ee35c11582d3/molecules-28-02172-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/57dd468f65de/molecules-28-02172-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/2d5ac496b76c/molecules-28-02172-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/6785a3385b3e/molecules-28-02172-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/67f0328db804/molecules-28-02172-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/0b97d8107503/molecules-28-02172-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/e4e7099faced/molecules-28-02172-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0666/10004670/ff1c2e629da1/molecules-28-02172-g010.jpg

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