Wang Wei, Tu Qiang, Chen Rongrong, Lv Pincang, Xu Yanqing, Xie Qian, Song Zhaojian, He Yuchi, Cai Detian, Zhang Xianhua
School of Life Sciences, Hubei University, Wuhan 430062, China.
Plants (Basel). 2022 Jan 4;11(1):132. doi: 10.3390/plants11010132.
Plant polyploidization is frequently associated with changes in nutrient contents. However, the possible contribution of metabolites to this change has not been investigated by characterizing the metabolite contents of diploid and tetraploid forms of rice ( L.). We compared the metabolites of a group of diploid-tetraploid brown rice and a group of diploid-tetraploid brown rice based on liquid chromatography-tandem mass spectrometry. In total, 401 metabolites were identified; of these, between the two diploid-tetraploid groups, 180 showed opposite expression trends, but 221 showed the same trends (147 higher abundance vs. 74 lower abundance). Hierarchical cluster analysis of differential metabolites between diploid and tetraploid species showed a clear grouping pattern, in which the expression abundance of lipids, amino acids and derivatives, and phenolic acids increased in tetraploids. Further analysis revealed that the lipids in tetraploid rice increased significantly, especially unsaturated fatty acids and phospholipids. This study provides further basis for understanding the changes in rice nutritional quality following polyploidization and may serve as a new theoretical reference for breeding eutrophic or functional rice varieties via polyploidization.
植物多倍体化常与营养成分的变化相关。然而，通过对水稻二倍体和四倍体形式的代谢物含量进行表征，尚未研究代谢物对这种变化的可能贡献。我们基于液相色谱 - 串联质谱法比较了一组二倍体 - 四倍体糙米和一组二倍体 - 四倍体糙米的代谢物。总共鉴定出401种代谢物；其中，在两个二倍体 - 四倍体组之间，180种表现出相反的表达趋势，但221种表现出相同的趋势（147种丰度较高，74种丰度较低）。二倍体和四倍体物种之间差异代谢物的层次聚类分析显示出明显的分组模式，其中四倍体中脂质、氨基酸及其衍生物和酚酸的表达丰度增加。进一步分析表明，四倍体水稻中的脂质显著增加，尤其是不饱和脂肪酸和磷脂。本研究为理解多倍体化后水稻营养品质的变化提供了进一步的依据，并可能为通过多倍体化培育富营养或功能性水稻品种提供新的理论参考。
