YE4对二肽基肽酶-4的抑制作用及潜在机制

Inhibitory Effect and Potential Mechanism of YE4 against Dipeptidyl Peptidase-4.

作者信息

Sha Jia, Song Jiajia, Huang Yechuan, Zhang Yuhong, Wang Hongwei, Zhang Yu, Suo Huayi

机构信息

College of Food Science, Southwest University, Chongqing 400715, China.

College of Bioengineering, Jingchu University of Technology, Jingmen 448000, China.

出版信息

Foods. 2021 Dec 29;11(1):80. doi: 10.3390/foods11010080.

This study investigated the inhibitory effect and mechanism of 12 LAB strains isolated from Chinese fermented foods on dipeptidyl peptidase-4 (DPP-4) using the Caco-2 cell model. The results showed that the inhibitory effect of cell-free extracts (CFEs) collected from each LAB strain on DPP-4 was higher than that of the cell-free excretory supernatants. The CFEs from YE4 (YE4-CFE) exhibited the strongest DPP-4 inhibitory activity (24.33% inhibition). Furthermore, YE4-CFE altered the TNF and MAPK signaling pathways. Additionally, the YE4-CFE ultrafiltration fraction (<3 kDa) displayed a similar DPP-4 inhibitory activity to YE4-CFE. UHPLC-MS/MS identified 19 compounds with a relative proportion of more than 1% in the <3 kDa fraction, and adenine, acetylcholine, and L-phenylalanine were the top three substances in terms of proportion. Altogether, the inhibitory effect of YE4-CFE on DPP-4 was associated with the TNF and MAPK signaling pathways, and with the high proportion of adenine, acetylcholine, and L-phenylalanine.

本研究利用Caco-2细胞模型，研究了从中国发酵食品中分离出的12株乳酸菌对二肽基肽酶-4（DPP-4）的抑制作用及其机制。结果表明，从各乳酸菌菌株收集的无细胞提取物（CFE）对DPP-4的抑制作用高于无细胞分泌上清液。来自YE4的CFE（YE4-CFE）表现出最强的DPP-4抑制活性（24.33%抑制率）。此外，YE4-CFE改变了TNF和MAPK信号通路。此外，YE4-CFE超滤级分（<3 kDa）对DPP-4的抑制活性与YE4-CFE相似。超高效液相色谱-串联质谱（UHPLC-MS/MS）鉴定出在<3 kDa级分中相对比例超过1%的19种化合物，腺嘌呤、乙酰胆碱和L-苯丙氨酸按比例位列前三。总之，YE4-CFE对DPP-4的抑制作用与TNF和MAPK信号通路有关，也与腺嘌呤、乙酰胆碱和L-苯丙氨酸的高比例有关。